LOCUS       sequence-69691-e         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression vector for an inducible mRFP1-tagged 
            constitutively active MEK5 mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Jensen KJ, Garmaroudi FS, Zhang J, Lin J, Boroomand S, Zhang M, Luo 
            Z, Yang D, Luo H, McManus BM, Janes KA
  TITLE     An ERK-p38 subnetwork coordinates host cell apoptosis and necrosis 
            during coxsackievirus B3 infection.
  JOURNAL   Cell Host Microbe. 2013 Jan 16;13(1):67-76. doi: 
            10.1016/j.chom.2012.11.009. Epub 2013 Jan 16.
  PUBMED    23332156
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chom.2012.11.009"; journalName: "Cell Host Microbe"; 
            date: "2013-01-16- 16"; volume: "13"; issue: "1"; pages: "67-76"
COMMENT     Sequence Label: pSLIK MEK5-DD-mRFP1 neo
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(178..795,796..801,802..837,838..840,841..876,877..879,
                     880..915,916..924)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
ORIGIN
        1 tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc
       61 agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagacg
      121 aagcttggta ccgagctcgg atctccaccc cgtaccggtc ctgcagtcga attcaccatg
      181 tctagactgg acaagagcaa agtcataaac ggagctctgg aattactcaa tggtgtcggt
      241 atcgaaggcc tgacgacaag gaaactcgct caaaagctgg gagttgagca gcctaccctg
      301 tactggcacg tgaagaacaa gcgggccctg ctcgatgccc tgccaatcga gatgctggac
      361 aggcatcata cccacttctg ccccctggaa ggcgagtcat ggcaagactt tctgcggaac
      421 aacgccaagt cataccgctg tgctctcctc tcacatcgcg acggggctaa agtgcatctc
      481 ggcacccgcc caacagagaa acagtacgaa accctggaaa atcagctcgc gttcctgtgt
      541 cagcaaggct tctccctgga gaacgcactg tacgctctgt ccgccgtggg ccactttaca
      601 ctgggctgcg tattggagga acaggagcat caagtagcaa aagaggaaag agagacacct
      661 accaccgatt ctatgccccc acttctgaga caagcaattg agctgttcga ccggcaggga
      721 gccgaacctg ccttcctttt cggcctggaa ctaatcatat gtggcctgga gaaacagcta
      781 aagtgcgaaa gcggcgggcc gaccgacgcc cttgacgatt ttgacttaga catgctccca
      841 gccgatgccc ttgacgactt tgaccttgat atgctgcctg ctgacgctct tgacgatttt
      901 gaccttgaca tgctccccgg gtaactaagt aaggatccgc ggccgc
//
