LOCUS       sequence-69703-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for GST-3xFlag-tagged ERK2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Bose AK, Janes KA
  TITLE     A high-throughput assay for phosphoprotein-specific phosphatase 
            activity in cellular extracts.
  JOURNAL   Mol Cell Proteomics. 2013 Mar;12(3):797-806. doi: 
            10.1074/mcp.O112.024059. Epub 2012 Dec 11.
  PUBMED    23233447
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/mcp.O112.024059"; journalName: "Mol Cell Proteomics"; date:
            "2013-03"; volume: "12"; issue: "3"; pages: "797-806"
COMMENT     Sequence Label: pGEX-4T-1 3xFlag-ERK2
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             14..85
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     regulatory      90..99
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gcgtggatct atggattaca aggatgacga cgataaggat tacaaggatg acgacgataa
       61 ggattacaag gatgacgacg ataagggatc catggcggcg gcggcggcgg gcccggagat
      121 ggtccgcggg caggtgttcg acgtggggcc gcgctacact aatctctcgt acatcggaga
      181 aggcgcctac ggcatggttt gttctgctta tgataatctc aacaaagttc gagttgctat
      241 caagaaaatc agtccttttg agcaccagac ctactgtcag agaaccctga gagagataaa
      301 aatcctactg cgcttcagac atgagaacat catcggcatc aatgacatca tccgggcacc
      361 aaccattgag cagatgaaag atgtatatat agtacaggac ctcatggaga cagatcttta
      421 caagctcttg aagacacagc acctcagcaa tgatcatatc tgctattttc tttatcagat
      481 cctgagagga ttaaagtata tacattcagc taatgttctg caccgtgacc tcaagccttc
      541 caacctcctg ctgaacacca cttgtgatct caagatctgt gactttggcc ttgcccgtgt
      601 tgcagatcca gaccatgatc atacagggtt cttgacagag tatgtagcca cgcgttggta
      661 cagagctcca gaaattatgt tgaattccaa gggttatacc aagtccattg atatttggtc
      721 tgtgggctgc atcctggcag agatgctatc caacaggcct atcttcccag gaaagcatta
      781 ccttgaccag ctgaatcaca tcctgggtat tcttggatct ccatcacagg aagatctgaa
      841 ttgtataata aatttaaaag ctagaaacta tttgctttct ctcccgcaca aaaataaggt
      901 gccgtggaac a
//
