LOCUS       sequence-69705-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for GST-3xFlag-tagged p38alpha.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Bose AK, Janes KA
  TITLE     A high-throughput assay for phosphoprotein-specific phosphatase 
            activity in cellular extracts.
  JOURNAL   Mol Cell Proteomics. 2013 Mar;12(3):797-806. doi: 
            10.1074/mcp.O112.024059. Epub 2012 Dec 11.
  PUBMED    23233447
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/mcp.O112.024059"; journalName: "Mol Cell Proteomics"; date:
            "2013-03"; volume: "12"; issue: "3"; pages: "797-806"
COMMENT     Sequence Label: pGEX-4T-1 3xFlag-p38alpha
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             14..85
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
ORIGIN
        1 gcgtggatct atggattaca aggatgacga cgataaggat tacaaggatg acgacgataa
       61 ggattacaag gatgacgacg ataagggatc catgtcgcag gagaggccca cgttctaccg
      121 gcaggagctg aacaagacca tctgggaggt gcccgaacga taccagaacc tgtccccggt
      181 gggctcgggc gcctatggct cggtgtgtgc tgcttttgat acaaagacgg ggcatcgtgt
      241 ggcagttaag aagctgtcga gaccgtttca gtccatcatt cacgccaaaa ggacctaccg
      301 agagttgcgt ctgctgaagc acatgaaaca cgaaaatgtg attggtctgt tggatgtgtt
      361 cacacccgca aggtcactgg aggaattcaa tgacgtgtac ctggtgaccc atctcatggg
      421 ggcggacctg aacaacatcg tgaagtgcca gaagctgacc gacgaccacg ttcagtttct
      481 catctaccag atcctccgag ggctgaagta tatacattcg gctgacataa ttcacaggga
      541 cctaaagccc agcaacctag ctgtgaacga agactgtgag ctcaagattc tggattttgg
      601 gctggctcgg cacactgatg atgagatgac aggctacgtg gctaccaggt ggtaccgagc
      661 cccagagatc atgctgaatt ggatgcacta taaccagaca gtggatattt ggtccgtggg
      721 ctgcatcatg gctgagctgt tgaccggaag aacgttgttt cctggtacag accatattga
      781 tcagttgaag ctcattttaa gactcgttgg aaccccaggg gctgagcttc tgaagaaaat
      841 ctcctcagag tctgcaagaa actacattca gtctctggcc ca
//
