LOCUS       sequence-69708-e         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for GST-3xHA-tagged constitutively 
            active MEK1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Bose AK, Janes KA
  TITLE     A high-throughput assay for phosphoprotein-specific phosphatase 
            activity in cellular extracts.
  JOURNAL   Mol Cell Proteomics. 2013 Mar;12(3):797-806. doi: 
            10.1074/mcp.O112.024059. Epub 2012 Dec 11.
  PUBMED    23233447
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/mcp.O112.024059"; journalName: "Mol Cell Proteomics"; date:
            "2013-03"; volume: "12"; issue: "3"; pages: "797-806"
COMMENT     Sequence Label: pGEX-4T-1 3xHA-MEK1-DD
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             14..94
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
ORIGIN
        1 gcgtggatct atgtacccat acgatgttcc agattacgct tacccatacg atgttccaga
       61 ttacgcttac ccatacgatg ttccagatta cgctggatcc atgcccaaga agaagccgac
      121 gcccatccag ctgaacccgg cccccgatgg ctcggcggtt aacgggacca gctcggccga
      181 gaccaacctg gaggccttgc agaagaagct ggaggagctg gagcttgacg agcagcagcg
      241 gaagcggctc gaggcctttc tgacgcagaa gcagaaggtg ggggaactga aggatgatga
      301 ctttgagaag atcagcgaac tgggagctgg caacggtgga gtggtcttca aggtctccca
      361 caagccatct ggcctggtta tggctagaaa gctgatccac ctggagatca aacccgcaat
      421 ccggaaccag atcatccggg agctgcaggt actgcacgag tgcaactccc cgtacatcgt
      481 gggcttctac ggggccttct acagcgacgg cgagatcagc atctgcatgg agcacatgga
      541 tggtgggtcc ttggatcaag ttctgaagaa agctggaaga attcctgagc aaattttagg
      601 aaaagttagc attgctgtga taaaaggcct gacctatctt cgggagaagc acaagattat
      661 gcacagagat gtcaagccat ccaacattct agtgaactca cgtggggaga tcaaactctg
      721 tgattttggg gtcagcgggc agctaattga cgatatggcc aacgatttcg tgggcacgag
      781 atcctacatg tcgcctgaga gactccaggg gactcactac tctgtgcagt cggacatctg
      841 gagcatgggg ctctctctgg tggagatggc agttgggaga taccccattc ctcctcctga
      901 tgccaaggag ctggagctac tgtttggatg ccatgtg
//
