LOCUS       sequence-69711-e         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for GST-3xHA-tagged constitutively 
            active MKK4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Bose AK, Janes KA
  TITLE     A high-throughput assay for phosphoprotein-specific phosphatase 
            activity in cellular extracts.
  JOURNAL   Mol Cell Proteomics. 2013 Mar;12(3):797-806. doi: 
            10.1074/mcp.O112.024059. Epub 2012 Dec 11.
  PUBMED    23233447
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/mcp.O112.024059"; journalName: "Mol Cell Proteomics"; date:
            "2013-03"; volume: "12"; issue: "3"; pages: "797-806"
COMMENT     Sequence Label: pGEX-4T-1 3xHA-MKK4-EE
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             14..94
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
ORIGIN
        1 gcgtggatct atgtacccat acgatgttcc agattacgct tacccatacg atgttccaga
       61 ttacgcttac ccatacgatg ttccagatta cgctggatcc atgcagggta aacgcaaagc
      121 actgaagttg aattttgcaa atccaccttt caaatctaca gcaaggttta ctctgaatcc
      181 caatcctaca ggagttcaaa acccacacat agagagactg agaacacaca gcattgagtc
      241 atcaggaaaa ctgaagatct cccctgaaca acactgggat ttcactgcag aggacttgaa
      301 agaccttgga gaaattggac gaggagctta tggttctgtc aacaaaatgg tccacaaacc
      361 aagtgggcaa ataatggcag ttaaaagaat tcggtcaaca gtggatgaaa aagaacaaaa
      421 acaacttctt atggatttgg atgtagtaat gcggagtagt gattgcccat acattgttca
      481 gttttatggt gcactcttca gagagggtga ctgttggatc tgtatggaac tcatgtctac
      541 ctcgtttgat aagttttaca aatatgtata tagtgtatta gatgatgtta ttccagaaga
      601 aattttaggc aaaatcactt tagcaactgt gaaagcacta aaccacttaa aagaaaactt
      661 gaaaattatt cacagagata tcaaaccttc caatattctt ctggacagaa gtggaaatat
      721 caagctctgt gacttcggca tcagtggaca gcttgtagac gagattgcca aggaaagaga
      781 tgctggctgt aggccataca tggcacctga aagaatagac ccaagcgcat cacgacaagg
      841 atatgatgtc cgctctgatg tctggagttt ggggatcaca ttgtatgagt tggccacagg
      901 ccgatttcct tatccaaagt ggaata
//
