LOCUS       sequence-69714-e         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for GST-3xHA-tagged constitutively 
            active MKK7a1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Bose AK, Janes KA
  TITLE     A high-throughput assay for phosphoprotein-specific phosphatase 
            activity in cellular extracts.
  JOURNAL   Mol Cell Proteomics. 2013 Mar;12(3):797-806. doi: 
            10.1074/mcp.O112.024059. Epub 2012 Dec 11.
  PUBMED    23233447
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/mcp.O112.024059"; journalName: "Mol Cell Proteomics"; date:
            "2013-03"; volume: "12"; issue: "3"; pages: "797-806"
COMMENT     Sequence Label: pGEX-4T-1 3xHA-MKK7a1-EE
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..27
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             31..111
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
ORIGIN
        1 aatcggaatc tggttccgcg tggatctatg tacccatacg atgttccaga ttacgcttac
       61 ccatacgatg ttccagatta cgcttaccca tacgatgttc cagattacgc tggatccatg
      121 ctggggctcc catcaacctt gttcacaccg cgcagtatgg agagcatcga gattgaccag
      181 aagctgcagg agatcatgaa gcagacaggg tacctgacta tcgggggcca gcgttatcag
      241 gcagaaatca atgacttgga gaacttgggt gagatgggca gtggtacctg tggtcaggtg
      301 tggaagatgc ggttccggaa gacaggccac atcattgctg ttaagcaaat gcggcgctct
      361 gggaacaagg aagagaataa gcgcattttg atggacctgg atgtagtact caagagccat
      421 gactgccctt acatcgttca gtgctttggc accttcatca ccaacacaga cgtctttatt
      481 gccatggagc tcatgggcac atgtgcagag aagctgaaga aacgaatgca gggccccatt
      541 ccagagcgaa tcctgggcaa gatgactgtg gcgattgtga aagcactgta ctatctgaag
      601 gagaagcatg gcgtcatcca tcgcgatgtc aaaccctcca acatcctgct agatgagcgg
      661 ggccagatca agctctgtga ctttggcatc agtggccgcc ttgttgacga aaaagccaaa
      721 gaacggagtg ctggctgtgc tgcctatatg gctcccgagc gcatcgaccc tccagatccc
      781 accaagcctg actatgacat ccgagctgat gtgtggagcc tgggcatctc actggtggag
      841 ctggcaacag gacagttccc ctataagaac tgcaagacgg actttgaggt cctcaccaaa
      901 gtcctacagg aagagccccc actcctgcct ggtcacatgg gcttctcagg ggacttccag
      961 tcatttgtca aagactgcct tactaa
//
