LOCUS       sequence-69722-e        1022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD2 
            efficiency..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai 
            QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D
  TITLE     Precise and reliable gene expression via standard transcription and 
            translation initiation elements.
  JOURNAL   Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 
            2013 Mar 10.
  PUBMED    23474465
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2404"; journalName: "Nat Methods"; date: "2013-04"; 
            volume: "10"; issue: "4"; pages: "354-60"
COMMENT     Sequence Label: pFAB3906
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(348..353,354..370,371..377)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     RBS             454..462
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcag
       61 atcatgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag tttactttgc
      121 agggcttccc aaccttacca gagggcgccc cagctggcaa ttccgacgtc gaattcaaaa
      181 gatcttaagt aagtaagagt atacgtatat cggctaataa cgtattaagg cgcttcggcg
      241 ccttttttta tgggggtatt ttcatcccaa tccacacgtc caacgcacag caaacaccac
      301 gtcgacccta tcagctgcgt gctttctatg agtcgttgct gcataacttg acaattaatc
      361 atccggctcg tataatgtgt ggagggccca agttcactta aaaaggagat caacaatgaa
      421 agcaattttc gtactgaaac atcttaatca tgctaaggag gttttctaat ggcgagtagc
      481 gaagacgtta tcaaagagtt catgcgtttc aaagttcgta tggaaggttc cgttaacggt
      541 cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct
      601 aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt gggacatcct gtccccgcag
      661 ttccagtacg gttccaaagc ttacgttaaa cacccggctg acatcccgga ctacctgaaa
      721 ctgtccttcc cggaaggttt caaatgggaa cgtgttatga acttcgaaga cggtggtgtt
      781 gttaccgtta cccaggactc ctccctgcaa gacggtgagt tcatctacaa agttaaactg
      841 cgtggtacca acttcccgtc cgacggtccg gttatgcaga aaaaaaccat gggttgggaa
      901 gcttccaccg aacgtatgta cccggaagac ggtgctctga aaggtgaaat caaaatgcgt
      961 ctgaaactga aagacggtgg tcactacgac gctgaagtta aaaccaccta catggctaaa
     1021 aa
//
