LOCUS       sequence-69735-e        1068 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BIOFAB reporter plasmid for measuring trp_att_L126 termination 
            efficiency.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai 
            QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D
  TITLE     Precise and reliable gene expression via standard transcription and 
            translation initiation elements.
  JOURNAL   Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 
            2013 Mar 10.
  PUBMED    23474465
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2404"; journalName: "Nat Methods"; date: "2013-04"; 
            volume: "10"; issue: "4"; pages: "354-60"
COMMENT     Sequence Label: pFAB801
FEATURES             Location/Qualifiers
     source          1..1068
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(377..395,396..401,402..418,419..424,425..450)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    377..395
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    402..420
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             464..475
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 acctgcgtgc aatccatctt gttcaatcat gcgaaacgat cctcatcctg tctcttgatc
       61 agatcatgat cccctgcgcc atcagatcct tggcggcaag aaagccatcc agtttacttt
      121 gcagggcttc ccaaccttac cagagggcgc cccagctggc aattccgacg tctagggata
      181 acagggtaat tacggcccca gaattcaaaa gatcttaagt aagtaagagt atacgtatat
      241 cggctaaaac gtattaaggc gcttcggcgc ctttttttat gggggtattt tcatcccaat
      301 ccacacgtcc aacgcacagc aaacaccacg tcgaccctat cagctgcgtg ctttctatga
      361 gtcgttgctg catatctccc tatcagtgat agagattgac atccctatca gtgatagaga
      421 tactgagcac atcagcagga cgcactgacc tttggaattc attaaagagg agaaaggtac
      481 catggcgagt agcgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg
      541 ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg
      601 tacccagacc gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat
      661 cctgtccccg cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc
      721 ggactacctg aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga
      781 agacggtggt gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta
      841 caaagttaaa ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac
      901 catgggttgg gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga
      961 aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac
     1021 ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccga
//
