LOCUS       sequence-69736-e         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BIOFAB reporter plasmid for measuring trp_att_L126 termination 
            efficiency.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai 
            QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D
  TITLE     Precise and reliable gene expression via standard transcription and 
            translation initiation elements.
  JOURNAL   Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 
            2013 Mar 10.
  PUBMED    23474465
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2404"; journalName: "Nat Methods"; date: "2013-04"; 
            volume: "10"; issue: "4"; pages: "354-60"
COMMENT     Sequence Label: pFAB801
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             23..34
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             complement(281..958)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
ORIGIN
        1 cttctccttt gctcatggta cctttctcct ctttaatgaa ttgattaacc ggaagaaggt
       61 caatcataaa ggccactctt gcgaatgacc ttgagtttgt ccctctatag tgagtccgcc
      121 tgtttgcatt gttattctct aattttgttc aaaaaaaagc ccgcttatta ggcgggctgg
      181 gtatctgatt gctttacgca tggtgaaatc acataagtaa agtgataatc ataaaggcca
      241 ctcgctagga gcgaccttga gtctatcact cagcagatta ttaagcaccg gtggagtgac
      301 gaccttcagc acgttcgtac tgttcaacga tggtgtagtc ttcgttgtgg gaggtgatgt
      361 ccagtttgat gtcggttttg taagcacccg gcagctgaac cggtttttta gccatgtagg
      421 tggttttaac ttcagcgtcg tagtgaccac cgtctttcag tttcagacgc attttgattt
      481 cacctttcag agcaccgtct tccgggtaca tacgttcggt ggaagcttcc caacccatgg
      541 tttttttctg cataaccgga ccgtcggacg ggaagttggt accacgcagt ttaactttgt
      601 agatgaactc accgtcttgc agggaggagt cctgggtaac ggtaacaaca ccaccgtctt
      661 cgaagttcat aacacgttcc catttgaaac cttccgggaa ggacagtttc aggtagtccg
      721 ggatgtcagc cgggtgttta acgtaagctt tggaaccgta ctggaactgc ggggacagga
      781 tgtcccaagc gaacggcagc ggaccacctt tggtaacttt cagtttagcg gtctgggtac
      841 cttcgtacgg acgaccttca ccttcacctt cgatttcgaa ctcgtgaccg ttaacggaac
      901 cttccatacg aactttgaaa cgcatgaact ctttgataac gtcttcgcta ctcgccatgg
//
