LOCUS       sequence-69751-e         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BIOFAB reporter plasmid for measuring T7 early termination 
            efficiency.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai 
            QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D
  TITLE     Precise and reliable gene expression via standard transcription and 
            translation initiation elements.
  JOURNAL   Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 
            2013 Mar 10.
  PUBMED    23474465
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2404"; journalName: "Nat Methods"; date: "2013-04"; 
            volume: "10"; issue: "4"; pages: "354-60"
COMMENT     Sequence Label: pFAB803
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             24..35
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      complement(152..179)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 tcttctcctt tgctcatggt acctttctcc tctttaatga attgattaac cggaagaagg
       61 tcaatcataa aggccactct tgcgaatgac cttgagtttg tccctctata gtgagtccgc
      121 ccttcttaaa cataaagtgt ctccttataa acgcagaaag gcccacccga aggtgagcca
      181 gtgtgattac attttctctt gagggttgta tcacataagt aaagtgataa tcataaaggc
      241 cactcgctag gagcgacctt gagtctatca ctcagcagat tattaagcac cggtggagtg
      301 acgaccttca gcacgttcgt actgttcaac gatggtgtag tcttcgttgt gggaggtgat
      361 gtccagtttg atgtcggttt tgtaagcacc cggcagctga accggttttt tagccatgta
      421 ggtggtttta acttcagcgt cgtagtgacc accgtctttc agtttcagac gcattttgat
      481 ttcacctttc agagcaccgt cttccgggta catacgttcg gtggaagctt cccaacccat
      541 ggtttttttc tgcataaccg gaccgtcgga cgggaagttg gtaccacgca gtttaacttt
      601 gtagatgaac tcaccgtctt gcagggagga gtcctgggta acggtaacaa caccaccgtc
      661 ttcgaagttc ataacacgtt cccatttgaa accttccggg aaggacagtt tcaggtagtc
      721 cgggatgtca gccgggtgtt taacgtaagc tttggaaccg tactggaact gcggggacag
      781 gatgtcccaa gcgaacggca gcggaccacc tttggtaact ttcagtttag cggtctgggt
      841 accttcgtac ggacgacctt caccttcacc ttcgatttc
//
