LOCUS       sequence-69753-e        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BIOFAB reporter plasmid for measuring T3 early termination 
            efficiency.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai 
            QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D
  TITLE     Precise and reliable gene expression via standard transcription and 
            translation initiation elements.
  JOURNAL   Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 
            2013 Mar 10.
  PUBMED    23474465
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2404"; journalName: "Nat Methods"; date: "2013-04"; 
            volume: "10"; issue: "4"; pages: "354-60"
COMMENT     Sequence Label: pFAB804
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(375..393,394..399,400..416,417..422,423..448)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    375..393
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    400..418
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             462..473
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcag
       61 atcatgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag tttactttgc
      121 agggcttccc aaccttacca gagggcgccc cagctggcaa ttccgacgtc tagggataac
      181 agggtaatta cggccccaga attcaaaaga tcttaagtaa gtaagagtat acgtatatcg
      241 gctaaaacgt attaaggcgc ttcggcgcct ttttttatgg gggtattttc atcccaatcc
      301 acacgtccaa cgcacagcaa acaccacgtc gaccctatca gctgcgtgct ttctatgagt
      361 cgttgctgca tatctcccta tcagtgatag agattgacat ccctatcagt gatagagata
      421 ctgagcacat cagcaggacg cactgacctt tggaattcat taaagaggag aaaggtacca
      481 tggcgagtag cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt
      541 ccgttaacgg tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta
      601 cccagaccgc taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc
      661 tgtccccgca gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg
      721 actacctgaa actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag
      781 acggtggtgt tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca
      841 aagttaaact gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca
      901 tgggttggga agcttccacc gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa
      961 tcaaaatgcg tctgaaactg aaagacggtg gtcactacga cgctgaagtt aaaacc
//
