LOCUS       sequence-69792-e        3431 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3431)
  TITLE     Series-14: N-terminal Tag E.coli T7 Expression Vectors with Biobrick
            POLYPROMOTER Restriction Sites
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET His6 Mocr TEV expression vector with BioBr...
FEATURES             Location/Qualifiers
     source          1..3431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      48..94
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      154..181
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        207..225
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             242..264
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             283..300
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             670..690
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     terminator      732..778
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      1945..1984
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      2015..2603
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2504..2523
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2757..2774
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2789..2928
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2854..2873)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3014..3036
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3030..3221)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ggcaccatgc atcgatcccg cgaaatgcgg ccgcttaatt aaggcatcaa ataaaacgaa
       61 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg agtccttcca
      121 gtctgggcga aagcgcggtc gatcaaatta agcagaaggc catcctgacg gatggccttt
      181 ttgcgtttct atcgatcccg cgaaattaat acgactcact ataggcccct ctagaaataa
      241 ttttgtttaa ctttaagaag gagatatacc atgggttctt ctcaccatca ccatcaccat
      301 ggttcttcta tgagcaacat gacatacaat aatgttttcg accacgcgta cgaaatgctt
      361 aaggagaaca tccgttatga tgacatacgg gatacagatg accttcacga cgcaattcat
      421 atggccgccg ataatgccgt ccctcactat tatgccgata ttcggtcagt tatggcatct
      481 gaaggtattg atctggaatt tgaggatagt ggcctcatgc cagatacgaa ggacgacatc
      541 agaattctgc aagcgcggat ctacgaacaa cttaccatcg atttatggga agatgcggaa
      601 gatctcctga atgaatacct ggaagaagtg gaggagtatg aggaagatga agaggggacg
      661 gggatcgagg aaaacctgta cttccaatcc aatattggaa gtggataact ggctgctgcc
      721 accgctgagc atagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct
      781 gaaaggagga actatatcaa gtcccgaagt agctgtcaga ctgctggcga ccgccgcaac
      841 tagtggcgtc atgcgtgccg tatggtcttt cgcctgtaga cgccctacaa gccgtactaa
      901 agtggaaaga cccgcaaaag gtatcgcgat cgccctgcag ggaactatat ccgactagta
      961 gcccgcctaa tgagcgggct tttttttaat tcccctattt gtttattttt ctaaatacat
     1021 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcgaaa aaggaagagt
     1081 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     1141 gtttttgctc acccagaaac gctcgtgaaa gtaaaagacg cagaggacca attgggggca
     1201 cgagtgggat acatagaact ggacttgaat agcggtaaaa tccttgagag ttttcgccct
     1261 gaagagcgtt ttccaatgat gagcactttc aaagttctgc tatgtggagc agtattatcc
     1321 cgtgtagatg cggggcaaga gcaactcgga cgacgaatac actattcgca gaatgacttg
     1381 gttgaatact ccccagtgac agaaaagcac cttacggacg gaatgacggt aagagaatta
     1441 tgtagtgccg ccataacgat gagtgataac actgcggcga acttacttct gacaaccatc
     1501 ggtggaccga aggaattaac cgcttttttg cacaatatgg gagaccatgt aactcgcctt
     1561 gaccgttggg aaccagaact gaatgaagcc ataccaaacg acgagcgaga caccacaatg
     1621 cctgcggcaa tggcaacaac attacgcaaa ctattaactg gcgaactact tactctggct
     1681 tcacggcaac aattaataga ctggcttgaa gcggataaag ttgcaggacc actactgcgt
     1741 tcggcacttc ctgctggctg gtttattgct gataaatctg gggcaggaga gcgtggttca
     1801 cggggtatca ttgccgcact tggaccagat ggtaagcctt cccgtatcgt agttatctac
     1861 acgacgggta gtcaggcaac tatggacgaa cgaaatagac agattgctga aataggggct
     1921 tcactgatta agcattggta aaccgataca attaaaggct ccttttggag cctttttttt
     1981 tggacggacc ggtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     2041 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     2101 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     2161 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     2221 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     2281 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     2341 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     2401 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     2461 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     2521 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     2581 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     2641 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     2701 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     2761 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt
     2821 gcggtatttc acaccgcaat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     2881 aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg
     2941 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     3001 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     3061 gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc
     3121 tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata
     3181 aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg
     3241 ggatttctgt tcatggggta atgataccga tgaaacgaga gaggatgctc acgatacggg
     3301 ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat
     3361 ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag
     3421 atgtaggtgt t
//
