LOCUS       sequence-69794-e        3071 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3071)
  TITLE     Series-14: N-terminal Tag E.coli T7 Expression Vectors with Biobrick
            POLYPROMOTER Restriction Sites
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET StrepII TEV expression vector with BioBric...
FEATURES             Location/Qualifiers
     source          1..3071
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      48..94
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      154..181
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        207..225
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             242..264
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             280..303
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             310..330
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     terminator      372..418
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      1585..1624
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      1655..2243
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2144..2163
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2397..2414
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2429..2568
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2494..2513)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2654..2676
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2670..2861)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ggcaccatgc atcgatcccg cgaaatgcgg ccgcttaatt aaggcatcaa ataaaacgaa
       61 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg agtccttcca
      121 gtctgggcga aagcgcggtc gatcaaatta agcagaaggc catcctgacg gatggccttt
      181 ttgcgtttct atcgatcccg cgaaattaat acgactcact ataggcccct ctagaaataa
      241 ttttgtttaa ctttaagaag gagatatacc atggctagct ggagccaccc gcagttcgaa
      301 aaaggcgccg aaaacctgta cttccaatcc aatattggaa gtggataact ggctgctgcc
      361 accgctgagc atagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct
      421 gaaaggagga actatatcaa gtcccgaagt agctgtcaga ctgctggcga ccgccgcaac
      481 tagtggcgtc atgcgtgccg tatggtcttt cgcctgtaga cgccctacaa gccgtactaa
      541 agtggaaaga cccgcaaaag gtatcgcgat cgccctgcag ggaactatat ccgactagta
      601 gcccgcctaa tgagcgggct tttttttaat tcccctattt gtttattttt ctaaatacat
      661 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcgaaa aaggaagagt
      721 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
      781 gtttttgctc acccagaaac gctcgtgaaa gtaaaagacg cagaggacca attgggggca
      841 cgagtgggat acatagaact ggacttgaat agcggtaaaa tccttgagag ttttcgccct
      901 gaagagcgtt ttccaatgat gagcactttc aaagttctgc tatgtggagc agtattatcc
      961 cgtgtagatg cggggcaaga gcaactcgga cgacgaatac actattcgca gaatgacttg
     1021 gttgaatact ccccagtgac agaaaagcac cttacggacg gaatgacggt aagagaatta
     1081 tgtagtgccg ccataacgat gagtgataac actgcggcga acttacttct gacaaccatc
     1141 ggtggaccga aggaattaac cgcttttttg cacaatatgg gagaccatgt aactcgcctt
     1201 gaccgttggg aaccagaact gaatgaagcc ataccaaacg acgagcgaga caccacaatg
     1261 cctgcggcaa tggcaacaac attacgcaaa ctattaactg gcgaactact tactctggct
     1321 tcacggcaac aattaataga ctggcttgaa gcggataaag ttgcaggacc actactgcgt
     1381 tcggcacttc ctgctggctg gtttattgct gataaatctg gggcaggaga gcgtggttca
     1441 cggggtatca ttgccgcact tggaccagat ggtaagcctt cccgtatcgt agttatctac
     1501 acgacgggta gtcaggcaac tatggacgaa cgaaatagac agattgctga aataggggct
     1561 tcactgatta agcattggta aaccgataca attaaaggct ccttttggag cctttttttt
     1621 tggacggacc ggtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     1681 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     1741 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     1801 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     1861 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     1921 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     1981 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     2041 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     2101 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     2161 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     2221 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     2281 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     2341 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     2401 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt
     2461 gcggtatttc acaccgcaat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     2521 aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg
     2581 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     2641 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     2701 gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc
     2761 tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata
     2821 aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg
     2881 ggatttctgt tcatggggta atgataccga tgaaacgaga gaggatgctc acgatacggg
     2941 ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat
     3001 ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag
     3061 atgtaggtgt t
//
