LOCUS       sequence-69825-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vitro transcription of golden gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Jao LE, Wente SR, Chen W
  TITLE     Efficient multiplex biallelic zebrafish genome editing using a 
            CRISPR nuclease system.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Aug 5.
  PUBMED    23918387
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Aug 5."
COMMENT     Sequence Label: pT7goldRNA
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        37..112
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(140..157)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(140..156)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(149..171)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        376..480
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(699..718)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aatacgactc actataggtc tctcgcagga tgttgcgttt tagagctaga aatagcaagt
       61 taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcggatcccc
      121 gggtaccgag ctcgaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
      181 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
      241 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc
      301 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata aggtggcact
      361 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
      421 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
      481 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
      541 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
      601 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
      661 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
      721 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
      781 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaat
//
