LOCUS       sequence-69851-e         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  hypoxia-responsive enhanced green fluorescent protein (EGFP)-based 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Vordermark D, Shibata T, Brown JM
  TITLE     Green fluorescent protein is a suitable reporter of tumor hypoxia 
            despite an oxygen requirement for chromophore formation.
  JOURNAL   Neoplasia. 2001 Nov-Dec;3(6):527-34.
  PUBMED    11774035
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neoplasia. 
            2001 Nov-Dec"; volume: "3(6)"; pages: "527-34"
COMMENT     Sequence Label: 5HRE/GFP
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(54..78)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(75..113)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        395..499
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(718..737)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ccttgctcac catggtggct ttaccaacag taccggaatg ccaagcttct agtgatctga
       61 cggttcacta aacgagctct gcttatatag acctcccacc gtacacgcct accagatctc
      121 gacaagagga cctgttggag cccacgtatg cactgtggct cgacaagagg acctgttgga
      181 gcccacgtat gcactgtggc tcgacaagag gacctgttgg agcccacgta tgcactgtgg
      241 ctcgacaaga ggacctgttg gagcccacgt atgcactgtg gctcgacaag aggacctgtt
      301 ggagcccacg tatgcactgt ggctcgagaa aagctcggta ccgatctccc gatccgtcga
      361 tctgacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
      421 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
      481 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
      541 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
      601 tgaagatcag ttgggtgccc gagtgggtta catcgaactg gatctcaaca gcggtaagat
      661 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
      721 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
      781 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
      841 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
      901 cttacttctg aca
//
