LOCUS       sequence-69876-e         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD13 
            efficiency..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai 
            QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D
  TITLE     Precise and reliable gene expression via standard transcription and 
            translation initiation elements.
  JOURNAL   Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 
            2013 Mar 10.
  PUBMED    23474465
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2404"; journalName: "Nat Methods"; date: "2013-04"; 
            volume: "10"; issue: "4"; pages: "354-60"
COMMENT     Sequence Label: pFAB3989
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(337..342,343..359,360..366)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 ccatcttgtt caatcatgcg aaacgatcct catcctgtct cttgatcaga tcatgatccc
       61 ctgcgccatc agatccttgg cggcaagaaa gccatccagt ttactttgca gggcttccca
      121 accttaccag agggcgcccc agctggcaat tccgacgtcg aattcaaaag atcttaagta
      181 agtaagagta tacgtatatc ggctaataac gtattaaggc gcttcggcgc ctttttttat
      241 gggggtattt tcatcccaat ccacacgtcc aacgcacagc aaacaccacg tcgaccctat
      301 cagctgcgtg ctttctatga gtcgttgctg cataacttga caattaatca tccggctcgt
      361 ataatgtgtg gagggcccaa gttcacttaa aaaggagatc aacaatgaaa gcaattttcg
      421 tactgaaaca tcttaatcat gcaatggagg ctttctaatg gcgagtagcg aagacgttat
      481 caaagagttc atgcgtttca aagttcgtat ggaaggttcc gttaacggtc acgagttcga
      541 aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc cagaccgcta aactgaaagt
      601 taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg tccccgcagt tccagtacgg
      661 ttccaaagct tacgttaaac acccggctga catcccggac tacctgaaac tgtccttccc
      721 ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac ggtggtgttg ttaccgttac
      781 ccaggactcc tccctgcaag acggtgagtt catctacaaa gttaaactgc gtggtaccaa
      841 cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg ggttgggaag cttccaccga
      901 acgtatgtac ccggaagacg gtgctctga
//
