LOCUS       sequence-69883-e         762 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This is a Gateway destination vector for secreted expression in 
            Drosophila cell culture by induction via CuSO4. Inserts will be 
            C-terminally tagged with Fc, V5 and hexahistidine tags. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Ozkan E, Carrillo RA, Eastman CL, Weiszmann R, Waghray D, Johnson 
            KG, Zinn K, Celniker SE, Garcia KC
  TITLE     An Extracellular Interactome of Immunoglobulin and LRR Proteins 
            Reveals Receptor-Ligand Networks.
  JOURNAL   Cell. 2013 Jul 3;154(1):228-39. doi: 10.1016/j.cell.2013.06.006.
  PUBMED    23827685
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.06"; journalName: "Cell"; date: "2013-07-3- 3";
            volume: "154"; issue: "1"; pages: "228-39"
COMMENT     Sequence Label: pECIA2
FEATURES             Location/Qualifiers
     source          1..762
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(69..75,76..93,94..99))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(124..248)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     sig_peptide     complement(258..311)
                     /codon_start=1
                     /product="Drosophila BiP signal sequence"
                     /label=BiP signal sequence
                     /translation="MKLCILLAVVAFVGLSLG"
     promoter        complement(329..755)
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
ORIGIN
        1 gatttttttc tccattttag cttccttagc tcctgaaaat ctcgacggat cctaactcaa
       61 aatccacaca ttatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgcggc
      121 cgccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa
      181 atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc ttttttgtac
      241 aaacttgttg atgatctccc gagcgagagg ccaacaaagg ccacgacggc cagtaatatg
      301 cataacttca tattgagatc ggatcccccc tttagttgca ctgagatgat tcacttgatt
      361 ttgctgctga ccacaactga tgcacattgg ccagatgctc tcggttttat acattttgga
      421 aacgcggctt tacacacggg tctttcgttc gattcacctc ttttgcacac gccggccggc
      481 tccaaattcg agtggagacg tgtgcagaag cttttgttct gcccgcgaag aattggttcg
      541 gttctccact ttggggactt gtatgggatg caaagctctt ggtggggcac atggggcatg
      601 cgcaggcact cttacttgcg taccacatat gtatgggggt ggcggtgggc ggtggggggc
      661 cggagttctg ggtctcttat cggaaccaga ggataggacg gcctgcagca aagagctagt
      721 cacatcgggc accacatcct gtcctgcaac gaattaattc ac
//
