LOCUS       sequence-69886-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This is a Gateway destination vector for secreted expression in 
            Drosophila cell culture by induction via CuSO4. Inserts will be 
            C-terminally tagged with Fc, V5 and hexahistidine tags. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Ozkan E, Carrillo RA, Eastman CL, Weiszmann R, Waghray D, Johnson 
            KG, Zinn K, Celniker SE, Garcia KC
  TITLE     An Extracellular Interactome of Immunoglobulin and LRR Proteins 
            Reveals Receptor-Ligand Networks.
  JOURNAL   Cell. 2013 Jul 3;154(1):228-39. doi: 10.1016/j.cell.2013.06.006.
  PUBMED    23827685
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.06"; journalName: "Cell"; date: "2013-07-3- 3";
            volume: "154"; issue: "1"; pages: "228-39"
COMMENT     Sequence Label: pECIA2
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    131..265
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(168..187)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     222..241
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     298..315
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(338..355)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(365..406)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 agcttgcatg cctgcaggtc gactgatcat aatcagccat accacatttg tagaggtttt
       61 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
      121 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
      181 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
      241 caatgtatct tatcatgtct ggatcctcga tcgaggctga tcagcgagct caggccttag
      301 aaggcacagt cgaggctgat cagcgggttt aaactcaatg gtgatggtga tgatgaccgg
      361 tacgcgtaga atcgagaccg aggagagggt tagggatagg cttaccttcg aagggccctc
      421 tagatttacc cggagacagg gagaggctct tctgcgtgta gtggttgtgc agaccctcat
      481 gcatcacgga gcatgagaag acgttcccct gctgccacct gctcttgtcc acggtgagct
      541 tgctgtagag gaagaaggag ccgtcggagt ccagcacggg aggcgtggtc ttgtagttgt
      601 tctccggctg cccattgctc tcccactcca cggcgatgtc gctgggatag aagcctttga
      661 ccaggcaggt caggctgacc tggttcttgg tcatctcctc ccgggatggg ggcagggtgt
      721 acacctgtgg ttctcggggc tgccctttgg ctttggagat ggttttctcg atgggggctg
      781 ggagggcttt gttggagacc ttgcacttgt actccttgcc attcagccag tcctggtgca
      841 ggac
//
