LOCUS       sequence-69907-e         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependant TVA-mCherry fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Miyamichi K, Shlomai-Fuchs Y, Shu M, Weissbourd BC, Luo L, Mizrahi A
  TITLE     Dissecting local circuits: parvalbumin interneurons underlie broad 
            feedback control of olfactory bulb output.
  JOURNAL   Neuron. 2013 Dec 4;80(5):1232-45. doi: 10.1016/j.neuron.2013.08.027.
            Epub 2013 Nov 14.
  PUBMED    24239125
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2013.08.027"; journalName: "Neuron"; date: 
            "2013-12-4- 4"; volume: "80"; issue: "5"; pages: "1232-45"
COMMENT     Sequence Label: CAG-Flex-TCB
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    25..613
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             131..142
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     540..560
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    637..670
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    741..774
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      781..790
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cnnnnnnnga tctacggtat cgatgcgggg aggcggccca aagggagatc cgactcgtct
       61 gagggcgaag gcgaagacgc ggaagaggcc gcagagccgg cagcaggccg cgggaaggaa
      121 ggtccgctgg attgagggcc gaagggacgt agcagaagga cgtcccgcgc agaatccagg
      181 tggcaacaca ggcgagcagc caaggaaagg acgatgattt ccccgacaac accacggaat
      241 tgtcagtgcc caacagccga gcccctgtcc agcagcgggc aaggcaggcg gcgatgagtt
      301 ccgccgtggc aatagggagg gggaaagcga aagtcccgga aaggagctga caggtggtgg
      361 caatgcccca accagtgggg gttgcgtcag caaacacagt gcacaccacg ccacgttgcc
      421 tgacaacggg ccacaactcc tcataaagag acagcaacca ggatttatac aaggaggaga
      481 aaatgaaagc catacgggaa gcaatagcat gatacaaagg cattaaagca gcgtatccac
      541 atagcgtaaa aggagcaaca tagttaagaa taccagtcaa tctttcacaa attttgtaat
      601 ccagaggttg attatcgatc ggccgcatat aagcttataa cttcgtataa tgtatgctat
      661 acgaagttat gggtcgatgg tgagatctgg actagagggt cgatggtgat gcttggatca
      721 aaataggaag aatcccaatg ataacttcgt ataaagtatc ctatacgaag ttataggcgc
      781 gccaccatgg cgcggctgct gcccgcgctg ctgctgctgc tgctgcccgg taacgtgacc
      841 ggtaacgggt ccggtaacgg ttctttgtcc cgttgccccc ccggtcagtt ccgctgctcg
      901 ganccgcccg gngcccacgg gggagtgnta cccgcnggac tggctnnncg acgggannnc
      961 cccgnctgcg acgacgggcg gnncnantgn nncnnnngg
//
