LOCUS       sequence-69915-e         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD20 
            efficiency..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai 
            QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D
  TITLE     Precise and reliable gene expression via standard transcription and 
            translation initiation elements.
  JOURNAL   Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 
            2013 Mar 10.
  PUBMED    23474465
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2404"; journalName: "Nat Methods"; date: "2013-04"; 
            volume: "10"; issue: "4"; pages: "354-60"
COMMENT     Sequence Label: pFAB3996
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(339..344,345..361,362..368)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca gatcatgatc
       61 ccctgcgcca tcagatcctt ggcggcaaga aagccatcca gtttactttg cagggcttcc
      121 caaccttacc agagggcgcc ccagctggca attccgacgt cgaattcaaa agatcttaag
      181 taagtaagag tatacgtata tcggctaata acgtattaag gcgcttcggc gccttttttt
      241 atgggggtat tttcatccca atccacacgt ccaacgcaca gcaaacacca cgtcgaccct
      301 atcagctgcg tgctttctat gagtcgttgc tgcataactt gacaattaat catccggctc
      361 gtataatgtg tggagggccc aagttcactt aaaaaggaga tcaacaatga aagcaatttt
      421 cgtactgaaa catcttaatc atgctgagga aagtttctaa tggcgagtag cgaagacgtt
      481 atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg tcacgagttc
      541 gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc taaactgaaa
      601 gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca gttccagtac
      661 ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa actgtccttc
      721 ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt tgttaccgtt
      781 acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact gcgtggtacc
      841 aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga agcttccacc
      901 gaacgtatgt acccggaa
//
