LOCUS       sequence-69916-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD21 
            efficiency..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai 
            QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D
  TITLE     Precise and reliable gene expression via standard transcription and 
            translation initiation elements.
  JOURNAL   Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 
            2013 Mar 10.
  PUBMED    23474465
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2404"; journalName: "Nat Methods"; date: "2013-04"; 
            volume: "10"; issue: "4"; pages: "354-60"
COMMENT     Sequence Label: pFAB3997
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(338..343,344..360,361..367)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcag atcatgatcc
       61 cctgcgccat cagatccttg gcggcaagaa agccatccag tttactttgc agggcttccc
      121 aaccttacca gagggcgccc cagctggcaa ttccgacgtc gaattcaaaa gatcttaagt
      181 aagtaagagt atacgtatat cggctaataa cgtattaagg cgcttcggcg ccttttttta
      241 tgggggtatt ttcatcccaa tccacacgtc caacgcacag caaacaccac gtcgacccta
      301 tcagctgcgt gctttctatg agtcgttgct gcataacttg acaattaatc atccggctcg
      361 tataatgtgt ggagggccca agttcactta aaaaggagat caacaatgaa agcaattttc
      421 gtactgaaac atcttaatca tgcgagggat ggtttctaat ggcgagtagc gaagacgtta
      481 tcaaagagtt catgcgtttc aaagttcgta tggaaggttc cgttaacggt cacgagttcg
      541 aaatcgaagg tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag
      601 ttaccaaagg tggtccgctg ccgttcgctt gggacatcct gtccccgcag ttccagtacg
      661 gttccaaagc ttacgttaaa cacccggctg acatcccgga ctacctgaaa ctgtccttcc
      721 cggaaggttt caaatgggaa cgtgttatga acttcgaaga cggtggtgtt gttaccgtta
      781 cccaggactc ctccctgcaa gacggtgagt tcatctacaa agttaaactg cgtggtacca
      841 acttcccgtc cgacggtccg gttatgcaga aaaaaaccat gggttgggaa gcttccaccg
      901 aacgtatgta cccggaaga
//
