LOCUS       sequence-69921-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD24 
            efficiency..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai 
            QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D
  TITLE     Precise and reliable gene expression via standard transcription and 
            translation initiation elements.
  JOURNAL   Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 
            2013 Mar 10.
  PUBMED    23474465
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2404"; journalName: "Nat Methods"; date: "2013-04"; 
            volume: "10"; issue: "4"; pages: "354-60"
COMMENT     Sequence Label: pFAB4000
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(342..347,348..364,365..371)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga tcagatcatg
       61 atcccctgcg ccatcagatc cttggcggca agaaagccat ccagtttact ttgcagggct
      121 tcccaacctt accagagggc gccccagctg gcaattccga cgtcgaattc aaaagatctt
      181 aagtaagtaa gagtatacgt atatcggcta ataacgtatt aaggcgcttc ggcgcctttt
      241 tttatggggg tattttcatc ccaatccaca cgtccaacgc acagcaaaca ccacgtcgac
      301 cctatcagct gcgtgctttc tatgagtcgt tgctgcataa cttgacaatt aatcatccgg
      361 ctcgtataat gtgtggaggg cccaagttca cttaaaaagg agatcaacaa tgaaagcaat
      421 tttcgtactg aaacatctta atcatgcgat ggacggtttc taatggcgag tagcgaagac
      481 gttatcaaag agttcatgcg tttcaaagtt cgtatggaag gttccgttaa cggtcacgag
      541 ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg
      601 aaagttacca aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc gcagttccag
      661 tacggttcca aagcttacgt taaacacccg gctgacatcc cggactacct gaaactgtcc
      721 ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc
      781 gttacccagg actcctccct gcaagacggt gagttcatct acaaagttaa actgcgtggt
      841 accaacttcc cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc
      901 accgaacgta tgtacccgga aga
//
