LOCUS       sequence-69948-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BIOFAB reporter plasmid for measuring tonB termination efficiency.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai 
            QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D
  TITLE     Precise and reliable gene expression via standard transcription and 
            translation initiation elements.
  JOURNAL   Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 
            2013 Mar 10.
  PUBMED    23474465
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2404"; journalName: "Nat Methods"; date: "2013-04"; 
            volume: "10"; issue: "4"; pages: "354-60"
COMMENT     Sequence Label: pFAB805
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             8..19
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      complement(136..167)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 tggtaccttt ctcctcttta atgaattgat taaccggaag aaggtcaatc ataaaggcca
       61 ctcttgcgaa tgaccttgag tttgtccctc tatagtgagt ccgccgaaaa cctcgcgcct
      121 tacctgttga gtaatagtca aaagcctccg gtcggaggct tttgactttc tgcttactga
      181 atttcggtga tcacataagt aaagtgataa tcataaaggc cactcgctag gagcgacctt
      241 gagtctatca ctcagcagat tattaagcac cggtggagtg acgaccttca gcacgttcgt
      301 actgttcaac gatggtgtag tcttcgttgt gggaggtgat gtccagtttg atgtcggttt
      361 tgtaagcacc cggcagctga accggttttt tagccatgta ggtggtttta acttcagcgt
      421 cgtagtgacc accgtctttc agtttcagac gcattttgat ttcacctttc agagcaccgt
      481 cttccgggta catacgttcg gtggaagctt cccaacccat ggtttttttc tgcataaccg
      541 gaccgtcgga cgggaagttg gtaccacgca gtttaacttt gtagatgaac tcaccgtctt
      601 gcagggagga gtcctgggta acggtaacaa caccaccgtc ttcgaagttc ataacacgtt
      661 cccatttgaa accttccggg aaggacagtt tcaggtagtc cgggatgtca gccgggtgtt
      721 taacgtaagc tttggaaccg tactggaact gcggggacag gatgtcccaa gcgaacggca
      781 gcggaccacc tttggtaact ttcagtttag cggtctgggt accttcgtac ggacgacctt
      841 caccttcacc ttcga
//
