LOCUS       sequence-69954-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BIOFAB reporter plasmid for measuring ilvGEDA termination 
            efficiency.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai 
            QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D
  TITLE     Precise and reliable gene expression via standard transcription and 
            translation initiation elements.
  JOURNAL   Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 
            2013 Mar 10.
  PUBMED    23474465
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2404"; journalName: "Nat Methods"; date: "2013-04"; 
            volume: "10"; issue: "4"; pages: "354-60"
COMMENT     Sequence Label: pFAB816
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             8..19
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 tggtaccttt ctcctcttta atgaattgat taaccggaag aaggtcaatc ataaaggcca
       61 ctcttgcgaa tgaccttgag tttgtccctc tatagtgagt ccgcctgtct gctcctcggt
      121 tatgttttta aggtcaaaaa aaacccccgg acctttcggt gcgggggtct tagttcgtta
      181 aggcttgatc tctaatcaca taagtaaagt gataatcata aaggccactc gctaggagcg
      241 accttgagtc tatcactcag cagattatta agcaccggtg gagtgacgac cttcagcacg
      301 ttcgtactgt tcaacgatgg tgtagtcttc gttgtgggag gtgatgtcca gtttgatgtc
      361 ggttttgtaa gcacccggca gctgaaccgg ttttttagcc atgtaggtgg ttttaacttc
      421 agcgtcgtag tgaccaccgt ctttcagttt cagacgcatt ttgatttcac ctttcagagc
      481 accgtcttcc gggtacatac gttcggtgga agcttcccaa cccatggttt ttttctgcat
      541 aaccggaccg tcggacggga agttggtacc acgcagttta actttgtaga tgaactcacc
      601 gtcttgcagg gaggagtcct gggtaacggt aacaacacca ccgtcttcga agttcataac
      661 acgttcccat ttgaaacctt ccgggaagga cagtttcagg tagtccggga tgtcagccgg
      721 gtgtttaacg taagctttgg aaccgtactg gaactgcggg gacaggatgt cccaagcgaa
      781 cggcagcgga ccacctttgg taactttcag tttagcggtc tgggtacctt cgtacggacg
      841 accttcacct tcaccttcga tttcgaactc gtgaccgtta acggaacctt ccatac
//
