LOCUS       sequence-69956-e         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BIOFAB reporter plasmid for measuring RNAI termination efficiency.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai 
            QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D
  TITLE     Precise and reliable gene expression via standard transcription and 
            translation initiation elements.
  JOURNAL   Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 
            2013 Mar 10.
  PUBMED    23474465
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2404"; journalName: "Nat Methods"; date: "2013-04"; 
            volume: "10"; issue: "4"; pages: "354-60"
COMMENT     Sequence Label: pFAB807
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             8..19
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 tggtaccttt ctcctcttta atgaattgat taaccggaag aaggtcaatc ataaaggcca
       61 ctcttgcgaa tgaccttgag tttgtccctc tatagtgagt ccgccccttt ttttctgcgc
      121 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccaa cggtggtttg tttgccggat
      181 caagagctac caactcatca cataagtaaa gtgataatca taaaggccac tcgctaggag
      241 cgaccttgag tctatcactc agcagattat taagcaccgg tggagtgacg accttcagca
      301 cgttcgtact gttcaacgat ggtgtagtct tcgttgtggg aggtgatgtc cagtttgatg
      361 tcggttttgt aagcacccgg cagctgaacc ggttttttag ccatgtaggt ggttttaact
      421 tcagcgtcgt agtgaccacc gtctttcagt ttcagacgca ttttgatttc acctttcaga
      481 gcaccgtctt ccgggtacat acgttcggtg gaagcttccc aacccatggt ttttttctgc
      541 ataaccggac cgtcggacgg gaagttggta ccacgcagtt taactttgta gatgaactca
      601 ccgtcttgca gggaggagtc ctgggtaacg gtaacaacac caccgtcttc gaagttcata
      661 acacgttccc atttgaaacc ttccgggaag gacagtttca ggtagtccgg gatgtcagcc
      721 gggtgtttaa cgtaagcttt ggaaccgtac tggaactgcg gggacaggat gtcccaagcg
      781 aacggcagcg gaccaccttt ggtaactttc agtttagcgg tctgggtacc ttcgtacgga
      841 cgaccttcac cttcaccttc gatttcgaac tcgtgaccgt taacggaacc ttcc
//
