LOCUS       sequence-69959-e        1021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for Ca2+ inside primary cilia.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Su S, Phua SC, Derose R, Chiba S, Narita K, Kalugin PN, Katada T, 
            Kontani K, Takeda S, Inoue T
  TITLE     Genetically encoded calcium indicator illuminates calcium dynamics 
            in primary cilia.
  JOURNAL   Nat Methods. 2013 Sep 22. doi: 10.1038/nmeth.2647.
  PUBMED    24056873
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 22. doi: 10.1038/nmeth.2647."
COMMENT     Sequence Label: 5HT6-G-GECO1.0
FEATURES             Location/Qualifiers
     source          1..1021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(41..484)
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYIGAAEL
                     RHVMTNLGEKLTDEEVDEMIRVADIDGDGQVNYEEFVQMMTAK"
     primer_bind     600..619
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(986..1007)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gctcgagcgg ccgccngtgt gatgganatc tgcagaattc ttacttcgct gtcatcattt
       61 gtacaaactc ttcgtagttt acctgaccat ccccatcgat gtctgctacc ctgatcattt
      121 catcaacctc ttcatctgtt aacttctctc caaggtttgt catcacgtgg cgaagctctg
      181 ctgcgccgat gtagccattg ccgtccttat caaacacacg gaacgcttct ctaatttctt
      241 cttcactgtc tgtgtctttc atttttcttg ccatcattgt caggaactca gggaagtcga
      301 ttgtgccgtc accgtcggca tctacttcat tgatcatgtc ctgcagctct gcttctgtgg
      361 ggttctgccc cagagaccgc atcaccgtcc ccagctcctt ggttgttatc gtcccatccc
      421 cgtccttgtc aaatagggag aaagcctctt taaattctgc gatctgctct tcagtcagtt
      481 ggtcacgcgt gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga
      541 tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg
      601 tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggatgtag ccttcgggca
      661 tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg aagcactgca
      721 cgccgtaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc
      781 agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcaccctcg ccggacacgc
      841 tgaacttgtg gccgtttacg tcgccgtcca gctcgacctg gatgggcacc accccggtga
      901 acagctcctc gcccttgctc accatgctcc ctccggtacc gcccttgtac agctcgtcca
      961 tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct
     1021 c
//
