LOCUS       sequence-69960-e         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the Ping open reading frame 1 (ORF1) and transposase from 
            rice to allow mPing movement. The vector contains ORF1, transposase,
            mPing element and hph for hygromycin selection. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Hancock CN, Zhang F, Floyd K, Richardson AO, Lafayette P, Tucker D, 
            Wessler SR, Parrott WA
  TITLE     The rice miniature inverted repeat transposable element mPing is an 
            effective insertional mutagen in soybean.
  JOURNAL   Plant Physiol. 2011 Oct;157(2):552-62. doi: 10.1104/pp.111.181206. 
            Epub 2011 Aug 15.
  PUBMED    21844309
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.111.181206"; journalName: "Plant Physiol"; date: 
            "2011-10"; volume: "157"; issue: "2"; pages: "552-62"
COMMENT     Sequence Label: pPing
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(543..589)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(578..598)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 attcggccga ggataatgat aggagaagtg aaaagatgag aaagagaaaa agattagtct
       61 tcattgttat atctccttgg atcctctaga ttaggccagt cacaatggct agtgtcattg
      121 cacggctacc caaaatatta taccatcttc tctcaaatga aatcttttat gaaacaatcc
      181 ccacagtgga ggggtttcac tttgacgttt ccaagactaa gcaaagcatt taattgatac
      241 aagttgctgg gatcatttgt acccaaaatc cggcgcggcg cgggagaatg cggaggtcgc
      301 acggcggagg cggacgcaag agatccggtg aatgaaacga atcggcctca acgggggttt
      361 cactctgtta ccgaggactt ggaaacgacg ctgacgagtt tcaccaggat gaaactcttt
      421 ccttctctct catccccatt tcatgcaaat aatcattttt tattcagtct tacccctatt
      481 aaatgtgcat gacacaccag tgaaaccccc attgtgactg gccttatcta gagtcccccg
      541 tgttctctcc aaatgaaatg aacttcctta tatagaggaa gggtcttgcg aaggatagtg
      601 ggattgtgcg tcatccctta cgtcagtgga gatatcacat caatccactt gctttgaaga
      661 cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg
      721 accactgtcg gcagaggcat cttcaacgat ggcctttcct ttatcgcaat gatggcattt
      781 gtaggagcca ccttcctttt ccactatctt cacaataaag tgacagatag ctgggcaatg
      841 ga
//
