LOCUS       sequence-69962-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BIOFAB reporter plasmid for measuring BBa_B1006 termination 
            efficiency.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai 
            QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D
  TITLE     Precise and reliable gene expression via standard transcription and 
            translation initiation elements.
  JOURNAL   Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 
            2013 Mar 10.
  PUBMED    23474465
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2404"; journalName: "Nat Methods"; date: "2013-04"; 
            volume: "10"; issue: "4"; pages: "354-60"
COMMENT     Sequence Label: pFAB822
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             23..34
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             complement(233..910)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
ORIGIN
        1 cttctccttt gctcatggta cctttctcct ctttaatgaa ttgattaacc ggaagaaggt
       61 caatcataaa ggccactctt gcgaatgacc ttgagtttgt ccctctatag tgagtccgcc
      121 aaaaaaaacc ccgccctgtc aggggcgggg ttttttttta tcacataagt aaagtgataa
      181 tcataaaggc cactcgctag gagcgacctt gagtctatca ctcagcagat tattaagcac
      241 cggtggagtg acgaccttca gcacgttcgt actgttcaac gatggtgtag tcttcgttgt
      301 gggaggtgat gtccagtttg atgtcggttt tgtaagcacc cggcagctga accggttttt
      361 tagccatgta ggtggtttta acttcagcgt cgtagtgacc accgtctttc agtttcagac
      421 gcattttgat ttcacctttc agagcaccgt cttccgggta catacgttcg gtggaagctt
      481 cccaacccat ggtttttttc tgcataaccg gaccgtcgga cgggaagttg gtaccacgca
      541 gtttaacttt gtagatgaac tcaccgtctt gcagggagga gtcctgggta acggtaacaa
      601 caccaccgtc ttcgaagttc ataacacgtt cccatttgaa accttccggg aaggacagtt
      661 tcaggtagtc cgggatgtca gccgggtgtt taacgtaagc tttggaaccg tactggaact
      721 gcggggacag gatgtcccaa gcgaacggca gcggaccacc tttggtaact ttcagtttag
      781 cggtctgggt accttcgtac ggacgacctt caccttcacc ttcgatttcg aactcgtgac
      841 cgttaacgga accttccata cgaactttga aacgcatgaa ctctttgata acgtcttcgc
      901 tactcgccat ggtacctttc tc
//
