LOCUS       sequence-69963-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BIOFAB reporter plasmid for measuring BBa_B1006 U10 termination 
            efficiency.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai 
            QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D
  TITLE     Precise and reliable gene expression via standard transcription and 
            translation initiation elements.
  JOURNAL   Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 
            2013 Mar 10.
  PUBMED    23474465
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2404"; journalName: "Nat Methods"; date: "2013-04"; 
            volume: "10"; issue: "4"; pages: "354-60"
COMMENT     Sequence Label: pFAB1907
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             23..34
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             complement(235..912)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
ORIGIN
        1 cttctccttt gctcatggta cctttctcct ctttaatgaa ttgattaacc ggaagaaggt
       61 caatcataaa ggccactctt gcgaatgacc ttgagtttgt ccctctatag tgagtccgcc
      121 aaaaaaaaaa ccccgccctg tcaggggcgg ggtttttttt tatcacataa gtaaagtgat
      181 aatcataaag gccactcgct aggagcgacc ttgagtctat cactcagcag attattaagc
      241 accggtggag tgacgacctt cagcacgttc gtactgttca acgatggtgt agtcttcgtt
      301 gtgggaggtg atgtccagtt tgatgtcggt tttgtaagca cccggcagct gaaccggttt
      361 tttagccatg taggtggttt taacttcagc gtcgtagtga ccaccgtctt tcagtttcag
      421 acgcattttg atttcacctt tcagagcacc gtcttccggg tacatacgtt cggtggaagc
      481 ttcccaaccc atggtttttt tctgcataac cggaccgtcg gacgggaagt tggtaccacg
      541 cagtttaact ttgtagatga actcaccgtc ttgcagggag gagtcctggg taacggtaac
      601 aacaccaccg tcttcgaagt tcataacacg ttcccatttg aaaccttccg ggaaggacag
      661 tttcaggtag tccgggatgt cagccgggtg tttaacgtaa gctttggaac cgtactggaa
      721 ctgcggggac aggatgtccc aagcgaacgg cagcggacca cctttggtaa ctttcagttt
      781 agcggtctgg gtaccttcgt acggacgacc ttcaccttca ccttcgattt cgaactcgtg
      841 accgttaacg gaaccttcca tacgaacttt gaaacgcatg aactctttga taacgtcttc
      901 gctactcgcc atggtacctt tct
//
