LOCUS       sequence-69965-e         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BIOFAB reporter plasmid for measuring M13_central_T_linker_rrnD_T1 
            termination efficiency.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai 
            QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D
  TITLE     Precise and reliable gene expression via standard transcription and 
            translation initiation elements.
  JOURNAL   Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 
            2013 Mar 10.
  PUBMED    23474465
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2404"; journalName: "Nat Methods"; date: "2013-04"; 
            volume: "10"; issue: "4"; pages: "354-60"
COMMENT     Sequence Label: pFAB1902
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             8..19
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      complement(252..300)
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 tggtaccttt ctcctcttta atgaattgat taaccggaag aaggtcaatc ataaaggcca
       61 ctcttgcgaa tgaccttgag tttgtccctc tatagtgagt ccgcccggga gagtgttcac
      121 cgacaaacaa cagataaaac aaaaggccca gtcttccgac tgagcctttt gttttatttg
      181 atgtctggca gttcccatca gccggccgcc tgatcaatca gccggccgcc aataataatt
      241 ttttcatgtt gaaaatctcc aaaaaaaaag gctccaaaag gagcctttaa ttgtatcggt
      301 ttatcagctt gctttatcac ataagtaaag tgataatcat aaaggccact cgctaggagc
      361 gaccttgagt ctatcactca gcagattatt aagcaccggt ggagtgacga ccttcagcac
      421 gttcgtactg ttcaacgatg gtgtagtctt cgttgtggga ggtgatgtcc agtttgatgt
      481 cggttttgta agcacccggc agctgaaccg gttttttagc catgtaggtg gttttaactt
      541 cagcgtcgta gtgaccaccg tctttcagtt tcagacgcat tttgatttca cctttcagag
      601 caccgtcttc cgggtacata cgttcggtgg aagcttccca acccatggtt tttttctgca
      661 taaccggacc gtcggacggg aagttggtac cacgcagttt aactttgtag atgaactcac
      721 cgtcttgcag ggaggagtcc tgggtaacgg taacaacacc accgtcttcg aagttcataa
      781 cacgttccca tttgaaacct tccgggaagg acagtttc
//
