LOCUS       sequence-69966-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Tsr-Venus-Ubiquitin-CI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Hensel Z, Feng H, Han B, Hatem C, Wang J, Xiao J
  TITLE     Stochastic expression dynamics of a transcription factor revealed by
            single-molecule noise analysis.
  JOURNAL   Nat Struct Mol Biol. 2012 Aug;19(8):797-802. doi: 10.1038/nsmb.2336.
            Epub 2012 Jul 1.
  PUBMED    22751020
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2336";
            journalName: "Nat Struct Mol Biol"; date: "2012-08"; volume: "19"; 
            issue: "8"; pages: "797-802"
COMMENT     Sequence Label: pZH051
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             226..234
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 aatgtgtaag agcggggtta tttatgctgt tgtttttttg ttactcggga agggctttac
       61 ctcttccgca taaacgcttc catcagcgtt tatagttaaa aaaatctttc ggcctgcatg
      121 aatggccttg ttgatcgcgc tttgatatac gccgagatct ttagctgtct tggtttgccc
      181 aaagcgcatt gcataatctt tcagggttat gcgttgttcc atacaacctc cttagtacat
      241 gcaaccatta tcaccgccag aggtaaaata gtcaacacgc acggtgttag atatttatcc
      301 cttgcggtga tagatttaac tcgagcacac aggaaacagc tatgttaaaa cgtatcaaaa
      361 ttgtgaccag cttactgctg gttttggccg tttttggcct tttacaactg acatcaggcg
      421 gtctgttctt taatgcctta aagaatgaca aagaaaattt cactgtttta caaaccattc
      481 gccagcagca atccacgctg aatggcagct gggtcgcgtt gttgcagacg cgtaacaccc
      541 tcaaccgcgc gggtatccgc tacatgatgg atcagaataa tattggtagc ggttcaaccg
      601 ttgctgagct gatggagagt gccagtattt cgctgaaaca ggcggaaaaa aactgggcgg
      661 attacgaagc gttgccgcgt gacccgcgtc agagcaccgc cgcagcggca gagatcaaac
      721 gtaattacga tatttatcac aatgcgctgg cggagctgat ccaactgtta ggtgcaggca
      781 aaatcaacga gttctttgat cagccgaccc agggatatca ggacg
//
