LOCUS       sequence-69967-e         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BIOFAB reporter plasmid for measuring rpoC termination efficiency.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai 
            QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D
  TITLE     Precise and reliable gene expression via standard transcription and 
            translation initiation elements.
  JOURNAL   Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 
            2013 Mar 10.
  PUBMED    23474465
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2404"; journalName: "Nat Methods"; date: "2013-04"; 
            volume: "10"; issue: "4"; pages: "354-60"
COMMENT     Sequence Label: pFAB1910
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             21..32
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 tctcctttgc tcatggtacc tttctcctct ttaatgaatt gattaaccgg aagaaggtca
       61 atcataaagg ccactcttgc gaatgacctt gagtttgtcc ctctatagtg agtccgcctg
      121 acaaatgctc tttccctaaa ctccccccat aaaaaaaccc gccgaagcgg gtttttacgt
      181 tatttgcgga ttaacgatta catcacataa gtaaagtgat aatcataaag gccactcgct
      241 aggagcgacc ttgagtctat cactcagcag attattaagc accggtggag tgacgacctt
      301 cagcacgttc gtactgttca acgatggtgt agtcttcgtt gtgggaggtg atgtccagtt
      361 tgatgtcggt tttgtaagca cccggcagct gaaccggttt tttagccatg taggtggttt
      421 taacttcagc gtcgtagtga ccaccgtctt tcagtttcag acgcattttg atttcacctt
      481 tcagagcacc gtcttccggg tacatacgtt cggtggaagc ttcccaaccc atggtttttt
      541 tctgcataac cggaccgtcg gacgggaagt tggtaccacg cagtttaact ttgtagatga
      601 actcaccgtc ttgcagggag gagtcctggg taacggtaac aacaccaccg tcttcgaagt
      661 tcataacacg ttcccatttg aaaccttccg ggaaggacag tttcaggtag tccgggatgt
      721 cagccgggtg tttaacgtaa gctttggaac cgtactggaa ctgcggggac aggatgtccc
      781 aagcgaacgg cagcggacca cctttggtaa ctttcagttt agcggtctgg gtaccttcgt
      841 acggacgacc ttcaccttca ccttcgattt cgaactcgtg accgttaacg gaaccttcca
      901 tac
//
