LOCUS       sequence-69969-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BIOFAB reporter plasmid for measuring his_T_min_linker_crp_T_min 
            termination efficiency.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai 
            QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D
  TITLE     Precise and reliable gene expression via standard transcription and 
            translation initiation elements.
  JOURNAL   Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 
            2013 Mar 10.
  PUBMED    23474465
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2404"; journalName: "Nat Methods"; date: "2013-04"; 
            volume: "10"; issue: "4"; pages: "354-60"
COMMENT     Sequence Label: pFAB1935
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             17..28
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ctttgctcat ggtacctttc tcctctttaa tgaattgatt aaccggaaga aggtcaatca
       61 taaaggccac tcttgcgaat gaccttgagt ttgtccctct atagtgagtc cgccaaacaa
      121 aatggcgcgc taccaggtaa cgcgccaatc agccggccgc ctgatcaatc agccggccgc
      181 caataaaaaa gcccccggaa ggtgatcttc cgggggcatc acataagtaa agtgataatc
      241 ataaaggcca ctcgctagga gcgaccttga gtctatcact cagcagatta ttaagcaccg
      301 gtggagtgac gaccttcagc acgttcgtac tgttcaacga tggtgtagtc ttcgttgtgg
      361 gaggtgatgt ccagtttgat gtcggttttg taagcacccg gcagctgaac cggtttttta
      421 gccatgtagg tggttttaac ttcagcgtcg tagtgaccac cgtctttcag tttcagacgc
      481 attttgattt cacctttcag agcaccgtct tccgggtaca tacgttcggt ggaagcttcc
      541 caacccatgg tttttttctg cataaccgga ccgtcggacg ggaagttggt accacgcagt
      601 ttaactttgt agatgaactc accgtcttgc agggaggagt cctgggtaac ggtaacaaca
      661 ccaccgtctt cgaagttcat aacacgttcc catttgaaac cttccgggaa ggacagtttc
      721 aggtagtccg ggatgtcagc cgggtgttta acgtaagctt tggaaccgta ctggaactgc
      781 ggggacagga tgtcccaagc gaacggcagc ggaccacctt tggtaacttt cagtttagcg
      841 gtctgggtac cttcgtacgg acgaccttca ccttcacctt cgatttcgaa ctcgtgaccg
      901 ttaacggaac cttccatac
//
