LOCUS       sequence-69973-e         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of mCherry tagged hPMCA2, zb splice variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  TITLE     Strehler unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: mCherry-hPMCA2zb
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             36..743
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(181..199)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     449..468
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     regulatory      804..813
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aaccgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggaggata
       61 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
      121 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
      181 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
      241 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
      301 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
      361 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
      421 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
      481 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
      541 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
      601 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
      661 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
      721 gcggcatgga cgagctgtac aagtccggac tcagatctcg agctcaagct tcgaattctg
      781 cagtcgacgg taccgcggtg gcggccgcca tgg
//
