LOCUS       sequence-69974-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BIOFAB reporter plasmid for measuring ilvGEDA termination 
            efficiency.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai 
            QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D
  TITLE     Precise and reliable gene expression via standard transcription and 
            translation initiation elements.
  JOURNAL   Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 
            2013 Mar 10.
  PUBMED    23474465
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2404"; journalName: "Nat Methods"; date: "2013-04"; 
            volume: "10"; issue: "4"; pages: "354-60"
COMMENT     Sequence Label: pFAB816
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(351..369,370..375,376..392,393..398,399..424)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    351..369
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    376..394
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             438..449
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 tcatgcgaaa cgatcctcat cctgtctctt gatcagatca tgatcccctg cgccatcaga
       61 tccttggcgg caagaaagcc atccagttta ctttgcaggg cttcccaacc ttaccagagg
      121 gcgccccagc tggcaattcc gacgtctagg gataacaggg taattacggc cccagaattc
      181 aaaagatctt aagtaagtaa gagtatacgt atatcggcta aaacgtatta aggcgcttcg
      241 gcgccttttt ttatgggggt attttcatcc caatccacac gtccaacgca cagcaaacac
      301 cacgtcgacc ctatcagctg cgtgctttct atgagtcgtt gctgcatatc tccctatcag
      361 tgatagagat tgacatccct atcagtgata gagatactga gcacatcagc aggacgcact
      421 gacctttgga attcattaaa gaggagaaag gtaccatggc gagtagcgaa gacgttatca
      481 aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac gagttcgaaa
      541 tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta
      601 ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc cccgcagttc cagtacggtt
      661 ccaaagctta cgttaaacac ccggctgaca tcccggacta cctgaaactg tccttcccgg
      721 aaggtttcaa atgggaacgt gttatgaact tcgaagacgg tggtgttgtt accgttaccc
      781 aggactcctc cctgcaagac ggtgagttca tctacaaagt taaactgcgt ggtaccaact
      841 tcccgtccga cggtccggtt atgcaga
//
