LOCUS       sequence-69975-e         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of mCherry tagged hPMCA2, xb splice variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Paszty K, Caride AJ, Bajzer Z, Offord CP, Padanyi R, Hegedus L, 
            Varga K, Strehler EE, Enyedi A
  TITLE     Plasma membrane Ca(2)(+)-ATPases can shape the pattern of Ca(2)(+) 
            transients induced by store-operated Ca(2)(+) entry.
  JOURNAL   Sci Signal. 2015 Feb 17;8(364):ra19. doi: 10.1126/scisignal.2005672.
  PUBMED    25690014
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2015-02-17"; volume: "8(364):ra19. doi"; pages: " 
            10.1126/scisignal.2005672"
COMMENT     Sequence Label: mCherry-hPMCA2xb
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      31..40
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             37..744
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(182..200)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     450..469
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     regulatory      805..814
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aaccgtcaga atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggaggat
       61 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
      121 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
      181 gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
      241 cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg
      301 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
      361 gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag
      421 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg
      481 gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag
      541 aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc
      601 aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc
      661 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc
      721 ggcggcatgg acgagctgta caagtccgga ctcagatctc gagctcaagc ttcgaattct
      781 gcagtcgacg gtaccgcggt ggcggccgcc atgggtgaca tgaccaacag cgac
//
