LOCUS       sequence-69976-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of mCherry tagged hPMCA2, wb splice variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  TITLE     Strehler unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: mCherry-hPMCA2wb
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      32..41
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             38..745
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(183..201)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     451..470
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     regulatory      806..815
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tgaaccgtca gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagga
       61 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa
      121 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
      181 cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc
      241 tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt
      301 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
      361 cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa
      421 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg
      481 ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca
      541 gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc
      601 caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc
      661 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac
      721 cggcggcatg gacgagctgt acaagtccgg actcagatct cgagctcaag cttcgaattc
      781 tgcagtcgac ggtaccgcgg tggcggccgc catgggtgac atgaccaaca gcgac
//
