LOCUS       sequence-6998-ex       11645 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-regulated (Tet-off) lentiviral vector for transgene (mPGK 
            promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM 
            (Addgene#12247)) - 2nd generation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11645)
  AUTHORS   Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P
  TITLE     A versatile tool for conditional gene expression and knockdown.
  JOURNAL   Nat Methods. 2006 Feb;3(2):109-16. doi: 10.1038/nmeth846
  PUBMED    16432520
REFERENCE   2  (bases 1 to 11645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Methods"; date: "2006-02"; volume: "3"; issue: "2"; pages: 
            "109-16"
COMMENT     Sequence Label: pLVPT-rtTR-KRAB-2SM2
FEATURES             Location/Qualifiers
     source          1..11645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             12..645
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    692..817
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1310..1543
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1727..1771
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2038..2155
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2194..2693
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2229..2252)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2626..2645
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      2744..2753
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2750..2752,2753..2755,2756..3466)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2750..2752,2753..2755,2756..3466)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2795..2816)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3056..3075)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3403..3424
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3544..4111
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3562..4113
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3706..3723)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3933..3952
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4737..4757
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4860..5054
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     primer_bind     complement(5192..5208)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5193..5209)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    5235..5823
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5288..5308)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5706..5717)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    join(5961..5979,5980..6002,6003..6021,6022..6044,6045..6063,
                     6064..6086,6087..6105,6106..6128,6129..6147,6148..6170,
                     6171..6189,6190..6212,6213..6231)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     LTR             6270..6450
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6475..6493)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6476..6493)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6783..6802)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7016..7035)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7135..7157
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7195..7213)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7281..7385
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7386..7454,7455..8246)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7604..7623)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8417..9005
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8906..8925
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9159..9176
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9246..9266)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9251..9580
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9431..9566
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10714..10779
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10909..10929
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11354..11488
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11391..11410)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11445..11464
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg
       61 gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt
      121 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt
      181 agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat
      241 ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca
      301 cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa
      361 gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg
      421 agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag
      481 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
      541 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
      601 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
      661 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
      721 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
      781 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
      841 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
      901 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
      961 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     1021 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     1081 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     1141 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     1201 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     1261 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     1321 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
     1381 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     1441 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     1501 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     1561 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     1621 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     1681 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     1741 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     1801 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     1861 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     1921 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
     1981 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt
     2041 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     2101 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     2161 tcacgagact agcctcgacg atggtcgagt accgggtagg ggaggcgctt ttcccaaggc
     2221 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct
     2281 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc
     2341 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg
     2401 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca
     2461 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct
     2521 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg
     2581 ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc
     2641 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctcta
     2701 gcgggatcca ggcctaagct tacgcgtcct agcgctaccg gtcgccacca tggtgagcaa
     2761 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     2821 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     2881 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     2941 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     3001 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     3061 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     3121 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     3181 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     3241 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     3301 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     3361 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     3421 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtccg gactcagatc
     3481 tcgactagct agtagctagc tagctagtcg agctcaagct tcgaattccc gggctgcagg
     3541 aattccgccc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
     3601 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
     3661 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
     3721 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
     3781 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
     3841 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
     3901 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
     3961 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
     4021 catgctttac atgtgtttag tcgaggttaa aaaacgtcta ggccccccga accacgggga
     4081 cgtggttttc ctttgaaaaa cacgatgata ataccatggc tagactggac aagagcaaag
     4141 tcataaacgg cgctctggaa ttactcaatg gagtcggtat cgaaggcctg acgacaagga
     4201 aactcgctca aaagctggga gttgagcagc ctaccctgta ctggcacgtg aagaacaagc
     4261 gggccctgct cgatgccctg ccaatcgaga tgctggacag gcatcatacc cacttctgcc
     4321 ccctggaagg cgagtcatgg caagactttc tgcggaacaa cgccaagtca ttccgctgtg
     4381 ctctcctctc acatcgcgac ggggctaaag tgcatctcgg cacccgccca acagagaaac
     4441 agtacgaaac cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga
     4501 acgcactgta cgctctgtcc gccgtgggcc actttacact gggctgcgta ttggaggaac
     4561 aggagcatca agtagcaaaa gaggaaagag agacacctac caccgattct atgcccccac
     4621 ttctgagaca agcaattgag ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg
     4681 gcctggaact aatcatatgc ggattagaaa aacaacttaa atgtgaaagt gggtcgccaa
     4741 aaaagaagag aaaggtcgac ggcggtggtg ctttgtctcc tcagcactct gctgtcactc
     4801 aaggaagtat catcaagaac aaggagggca tggatgctaa gtcactaact gcctggtccc
     4861 ggacactggt gaccttcaag gatgtatttg tggacttcac cagggaggag tggaagctgc
     4921 tggacactgc tcagcagatc gtgtacagaa atgtgatgct ggagaactat aagaacctgg
     4981 tttccttggg ttatcagctt actaagccag atgtgatcct ccggttggag aagggagaag
     5041 agccctggct ggtggagaga gaaattcacc aagagaccca tcctgattca gagactgcat
     5101 ttgaaatcaa atcatcagtt taaggatctc gactagctag tagctagcta gctagtcgag
     5161 ctcaacttcg aattcgatat caagcttatc gcgataccgt cgacctcgac ctcgacctcg
     5221 actagtcata tgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     5281 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     5341 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     5401 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     5461 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     5521 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     5581 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     5641 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     5701 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     5761 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     5821 cgcatcggta cgtatggcca ggtaccttta agaccaatga cttacaaggc agctgtagat
     5881 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     5941 caagatctcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     6001 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     6061 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     6121 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     6181 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     6241 gtcggatcga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     6301 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     6361 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     6421 ccttttagtc agtgtggaaa atctctagca gcatctagaa ttaattccgt gtattctata
     6481 gtgtcaccta aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc
     6541 taacgacaat atgtacaagc ctaattgtgt agcatctggc ttactgaagc agaccctatc
     6601 atctctctcg taaactgccg tcagagtcgg tttggttgga cgaaccttct gagtttctgg
     6661 taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc agcagggtca tcgctagcca
     6721 gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg
     6781 cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag
     6841 cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat
     6901 ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg
     6961 ctgcttccta atgcaggagt cgcataaggg agagcgtcga atggtgcact ctcagtacaa
     7021 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     7081 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     7141 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     7201 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     7261 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     7321 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     7381 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     7441 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     7501 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     7561 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     7621 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     7681 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     7741 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     7801 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     7861 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     7921 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     7981 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     8041 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     8101 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     8161 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     8221 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     8281 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     8341 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     8401 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     8461 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     8521 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     8581 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     8641 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     8701 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     8761 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg
     8821 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     8881 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     8941 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     9001 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     9061 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     9121 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     9181 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     9241 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     9301 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     9361 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     9421 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     9481 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     9541 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttggac acaagacagg
     9601 cttgcgagat atgtttgaga ataccacttt atcccgcgtc agggagaggc agtgcgtaaa
     9661 aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat aatctcgcgc
     9721 aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg acacttcaca
     9781 tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt aaactcgcaa
     9841 gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt ggcggtctgt
     9901 accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc
     9961 cagctacgat cacgacaacc agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg
    10021 cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgcgg ttgcgattcg
    10081 tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct
    10141 ggttgatgac tatgttgttg atatcccgca agatacctgg attgaacagc cgtgggatat
    10201 gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc
    10261 gggcgttgtt ctttttaact tcaggcgggt tacaatagtt tccagtaagt attctggagg
    10321 ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc
    10381 ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg
    10441 cccttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct
    10501 ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc acgtattgtg atgagcgatg
    10561 ccgaacgtac cgacgatgat ttatacgata cggtgattgg ctaccgtggc ggcaactgga
    10621 tttatgagtg ggccccggat ctttgtgaag gaaccttact tctgtggtgt gacataattg
    10681 gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa
    10741 tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat
    10801 gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg
    10861 ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag
    10921 agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct
    10981 gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca
    11041 ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt
    11101 tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat
    11161 aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa
    11221 tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt
    11281 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
    11341 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
    11401 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
    11461 catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc
    11521 caaacttccc accccatacc ctattaccac tgccaattac ctagtggttt catttactct
    11581 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
    11641 ctaca
//
