LOCUS       sequence-69984-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BIOFAB reporter plasmid for measuring RNAI termination efficiency.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai 
            QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D
  TITLE     Precise and reliable gene expression via standard transcription and 
            translation initiation elements.
  JOURNAL   Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 
            2013 Mar 10.
  PUBMED    23474465
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2404"; journalName: "Nat Methods"; date: "2013-04"; 
            volume: "10"; issue: "4"; pages: "354-60"
COMMENT     Sequence Label: pFAB807
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(352..370,371..376,377..393,394..399,400..425)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    352..370
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    377..395
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             439..450
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 atcatgcgaa acgatcctca tcctgtctct tgatcagatc atgatcccct gcgccatcag
       61 atccttggcg gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag
      121 ggcgccccag ctggcaattc cgacgtctag ggataacagg gtaattacgg ccccagaatt
      181 caaaagatct taagtaagta agagtatacg tatatcggct aaaacgtatt aaggcgcttc
      241 ggcgcctttt tttatggggg tattttcatc ccaatccaca cgtccaacgc acagcaaaca
      301 ccacgtcgac cctatcagct gcgtgctttc tatgagtcgt tgctgcatat ctccctatca
      361 gtgatagaga ttgacatccc tatcagtgat agagatactg agcacatcag caggacgcac
      421 tgacctttgg aattcattaa agaggagaaa ggtaccatgg cgagtagcga agacgttatc
      481 aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa
      541 atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt
      601 accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt
      661 tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg
      721 gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc
      781 caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac
      841 ttcccgtccg acggtccgg
//
