LOCUS       sequence-69985-e        1093 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor plasmid for homologous recombination that expresses ECFP 
            consitutively using the Aedes aegypti polyubiquitin (PUb) promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1093)
  TITLE     Vosshall lab Aedes aegypti HR donor plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSL1180-HR-PUbECFP
FEATURES             Location/Qualifiers
     source          1..1093
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    68..202
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(92..111)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     146..165
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(343..363)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(373..1083,1084..1086,1087..1089))
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(415..436)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     764..783
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1023..1044
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 atgattacga attcgaatgg ccatgggacg tcgacctgag gtaattataa cccgggccct
       61 atatatggat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
      121 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
      181 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
      241 gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga
      301 ttatgatcag ttatctagat ccggtggatc ttacgggtcc tccaccttcc gctttttctt
      361 gggtcgagat ctcttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa
      421 ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcaggg cggactgggt
      481 gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg
      541 gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct tgaagttggc
      601 cttgatgccg ttcttctgct tgtcggcggt gatatagacg ttgtggctga tgtagttgta
      661 ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat
      721 gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc
      781 gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa
      841 gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac tgcacgcccc aggtcagggt
      901 ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt
      961 cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt
     1021 tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt
     1081 gctcaccatg cta
//
