LOCUS       sequence-69986-e         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BIOFAB reporter plasmid for measuring rrnB T1 termination 
            efficiency.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai 
            QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D
  TITLE     Precise and reliable gene expression via standard transcription and 
            translation initiation elements.
  JOURNAL   Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 
            2013 Mar 10.
  PUBMED    23474465
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2404"; journalName: "Nat Methods"; date: "2013-04"; 
            volume: "10"; issue: "4"; pages: "354-60"
COMMENT     Sequence Label: pFAB808
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(356..374,375..380,381..397,398..403,404..429)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    356..374
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    381..399
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             443..454
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca gatcatgatc ccctgcgcca
       61 tcagatcctt ggcggcaaga aagccatcca gtttactttg cagggcttcc caaccttacc
      121 agagggcgcc ccagctggca attccgacgt ctagggataa cagggtaatt acggccccag
      181 aattcaaaag atcttaagta agtaagagta tacgtatatc ggctaaaacg tattaaggcg
      241 cttcggcgcc tttttttatg ggggtatttt catcccaatc cacacgtcca acgcacagca
      301 aacaccacgt cgaccctatc agctgcgtgc tttctatgag tcgttgctgc atatctccct
      361 atcagtgata gagattgaca tccctatcag tgatagagat actgagcaca tcagcaggac
      421 gcactgacct ttggaattca ttaaagagga gaaaggtacc atggcgagta gcgaagacgt
      481 tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt
      541 cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa
      601 agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc agttccagta
      661 cggttccaaa gcttacgtta aacacccggc tgacatcccg gactacctga aactgtcctt
      721 cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt
      781 tacccaggac tcctccctgc aagacggtga gttcatctac aaagttaaac tgcgtggtac
      841 caacttcccg tccgacggtc cggttatgca gaaaaaaacc atgggttggg aagcttccac
      901 cgaacgtatg tacccggaag acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact
      961 gaaagacgg
//
