LOCUS       sequence-69999-e         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BIOFAB reporter plasmid for measuring his_T_min_linker_crp_T_min 
            termination efficiency.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai 
            QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D
  TITLE     Precise and reliable gene expression via standard transcription and 
            translation initiation elements.
  JOURNAL   Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 
            2013 Mar 10.
  PUBMED    23474465
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2404"; journalName: "Nat Methods"; date: "2013-04"; 
            volume: "10"; issue: "4"; pages: "354-60"
COMMENT     Sequence Label: pFAB1935
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(353..371,372..377,378..394,395..400,401..426)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    353..371
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    378..396
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             440..451
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 aatcatgcga aacgatcctc atcctgtctc ttgatcagat catgatcccc tgcgccatca
       61 gatccttggc ggcaagaaag ccatccagtt tactttgcag ggcttcccaa ccttaccaga
      121 gggcgcccca gctggcaatt ccgacgtcta gggataacag ggtaattacg gccccagaat
      181 tcaaaagatc ttaagtaagt aagagtatac gtatatcggc taaaacgtat taaggcgctt
      241 cggcgccttt ttttatgggg gtattttcat cccaatccac acgtccaacg cacagcaaac
      301 accacgtcga ccctatcagc tgcgtgcttt ctatgagtcg ttgctgcata tctccctatc
      361 agtgatagag attgacatcc ctatcagtga tagagatact gagcacatca gcaggacgca
      421 ctgacctttg gaattcatta aagaggagaa aggtaccatg gcgagtagcg aagacgttat
      481 caaagagttc atgcgtttca aagttcgtat ggaaggttcc gttaacggtc acgagttcga
      541 aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc cagaccgcta aactgaaagt
      601 taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg tccccgcagt tccagtacgg
      661 ttccaaagct tacgttaaac acccggctga catcccggac tacctgaaac tgtccttccc
      721 ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac ggtggtgttg ttaccgttac
      781 ccaggactcc tccctgcaag acggtgagtt catctacaaa gttaaactgc gtggtaccaa
      841 cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg ggttgggaag cttccaccga
      901 acgtatgtac ccggaagacg gtgctctgaa agg
//
