LOCUS       sequence-70003-e         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Yen HC, Xu Q, Chou DM, Zhao Z, Elledge SJ
  TITLE     Global protein stability profiling in mammalian cells.
  JOURNAL   Science. 2008 Nov 7;322(5903):918-23. doi: 10.1126/science.1160489.
  PUBMED    18988847
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2008-11-7- 7"; volume: "322"; issue: 
            "5903"; pages: "918-23"
COMMENT     Sequence Label: MSCV-CMV-DsRed-IRES-EGFP-DEST
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      159..168
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(295..313)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(345..365)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     563..582
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     757..780
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg
       61 ggaccgatcc agcctccgcg gccgggaacg gtgcattgga acggacctgc aggaattgat
      121 ccgcggccct agcgctaccg gactcagatc caccggtcgc caccatggcc tcctccgagg
      181 acgtcatcaa ggagttcatg cgcttcaagg tgcgcatgga gggctccgtg aacggccacg
      241 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
      301 tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttcc
      361 agtacggctc caaggtgtac gtgaagcacc ccgccgacat ccccgactac aagaagctgt
      421 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
      481 ccgtgaccca ggactcctcc ctgcaggacg gctccttcat ctacaaggtg aagttcatcg
      541 gcgtgaactt cccctccgac ggccccgtaa tgcagaagaa gactatgggc tgggaggcct
      601 ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagatccac aaggccctga
      661 agctgaagga cggcggccac tacctggtgg agttcaagtc catctacatg gccaagaagc
      721 ccgtgcagct gcccggctac tactacgtgg actccaagct ggacatcacc tcccacaacg
      781 aggactacac catc
//
