LOCUS       sequence-70004-e        1015 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET reporter for Ca2+ inside primary cilia.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Su S, Phua SC, Derose R, Chiba S, Narita K, Kalugin PN, Katada T, 
            Kontani K, Takeda S, Inoue T
  TITLE     Genetically encoded calcium indicator illuminates calcium dynamics 
            in primary cilia.
  JOURNAL   Nat Methods. 2013 Sep 22. doi: 10.1038/nmeth.2647.
  PUBMED    24056873
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 22. doi: 10.1038/nmeth.2647."
COMMENT     Sequence Label: 5HT6-YC3.60
FEATURES             Location/Qualifiers
     source          1..1015
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      59..68
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 acnnctgctt actggcttat cgaaattaat acgactcact atagggagac ccaagcttgc
       61 caccatggtt ccagagcccg gccctgtcaa cagtagcacc ccagcctggg gccccgggcc
      121 accgccagcc ccagggggca gcggctgggt ggccgctgcg ctgtgcgtgg tcatcgtgct
      181 gacggcagcc gccaattcgc tgctgatcgc gctcatctgc acgcagcccg cgctgcgcaa
      241 cacgtctaac ttcttcctgg tgtcgctctt cacgtcggac ctgatggtgg gattggtggt
      301 gatgccccca gccatgctga acgcgctgta tgggcgctgg gtactagctc gcggcctctg
      361 tctgctttgg accgccttcg acgtgatgtg ctgcagcgcc tccattctca acctctgcct
      421 catcagcctg gaccgctacc tgctcatcct ctcgccgctg cgctacaagc tgcgcatgac
      481 agccccgcgt gccctggcgc tcatcctggg tgcctggagc ctcgccgcgc tggcctcctt
      541 cctgcccctc ttgctgggct ggcatgaact gggcaaagct cgaacatctg ccccgggcca
      601 gtgccgccta ttggccagcc tgccttatgt cctcgtggcg tccggcgtca cctttttcct
      661 gccttcgggt gccatctgct tcacctactg caggatcttg ctggctgccc gcaagcaggc
      721 ggtgcaagtg gcctcgctca ccacgggcac ggctacggct ggccaggcct tggaaacctt
      781 gcaggtgccc aggacaccac gcccagggat ggagtctgct gacagtaggc gtctgaccac
      841 caagcatagc aggaaggcct tgaaggccag cctgaccctc ggcatcctgc tgagcatgtt
      901 ctttgtcacc tggctgccct tctttgtggc cagcatagct caggccgtat gtgactgcat
      961 ctctccaggt ctcttcgatg tcctcacatg gctgggatac tgtaatagca ccatg
//
