LOCUS       sequence-70008-e         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Yen HC, Xu Q, Chou DM, Zhao Z, Elledge SJ
  TITLE     Global protein stability profiling in mammalian cells.
  JOURNAL   Science. 2008 Nov 7;322(5903):918-23. doi: 10.1126/science.1160489.
  PUBMED    18988847
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2008-11-7- 7"; volume: "322"; issue: 
            "5903"; pages: "918-23"
COMMENT     Sequence Label: MSCV-CMV-DsRed-IRES-EGFP-DEST
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(101..225)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(256..672)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(272..288)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(304..326)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa
       61 atgtcaggct cccttataca cagccagtct gcaggtcgac catagtgact ggatatgttg
      121 tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt
      181 tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggtgatcg gcctagataa
      241 ctgagatcta attccggcgc ctagagaagg agtgagggct ggataaaggg aggatcgagg
      301 cggggtcgaa cgaggaggtt caagggggag agacggggcg gatggaggaa gaggaggcgg
      361 aggcttaggg tgtacaaagg gcttgaccca gggagggggg tcaaaagcca aggcttccca
      421 ggtcacgatg taggggacct ggtctgggtg tccatgcggg ccaggtgaaa agaccttgat
      481 cttaacctgg gtgatgaggt ctcggttaaa ggtgccgtct cgcggccatc cgacgttaaa
      541 ggttggccat tctgcagagc agaaggtaac ccaacgtctc ttcttgacat ctaccgactg
      601 gttgtgagcg atccgctcga catctttcca gtgacctaag gtcaaactta agggagtggt
      661 aacagtctgg ccctaatttt cagacaaata cagaaacaca gtcagacaga gacaacacag
      721 aacgatgctg cagcgctgca gcagacaaga cgcgcggctt cggttccaaa ccgaaagcaa
      781 aaa
//
