LOCUS       sequence-70011-e         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  wt esaR promoter in pCS26.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Shong J, Huang YM, Bystroff C, Collins CH
  TITLE     Directed evolution of the quorum-sensing regulator EsaR for 
            increased signal sensitivity.
  JOURNAL   ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub
            2013 Feb 6.
  PUBMED    23363022
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/cb3006402";
            journalName: "ACS Chem Biol"; date: "2013-04-19- 19"; volume: "8"; 
            issue: "4"; pages: "789-95"
COMMENT     Sequence Label: pCS-PesaRlux
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     197..215
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcag atcttgatcc
       61 cctgcgccat cagatccttg gcggcaagaa agccatccag tttactttgc agggcttccc
      121 aaccttacca gagggcgccc cagctggcaa ttccgacgtc taagaaacca ttattatcat
      181 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcacc tcgaggcaga
      241 ttgagtaacc gtgaatgttt gtacaaatgt ttcaaagatg ttactatgag tgtcccggcc
      301 agcatcactt tatattttgt gacgtctggc cggacgtttt ccctagtgtt ggctgtttta
      361 gcgacctggc cgtacaggtc aggttttttt ttaccgctaa acaactgaag ccattgtaac
      421 ctctgaatga ttcattgtaa gttactctta agtatcatct tgcctgtact atagtgcagg
      481 ttaagtccac gttaagtaaa agaagcagcg gatcctctag ttgcggccgc aaatggatgg
      541 caaatatgac taaaaaaatt tcattcatta ttaacggcca ggttgaaatc tttcccgaaa
      601 gtgatgattt agtgcaatcc attaattttg gtgataatag tgtttacctg ccaatattga
      661 atgactctca tgtaaaaaac attattgatt gtaatggaaa taacgaatta cggttgcata
      721 acattgtcaa ttttctctat acggtagggc aaagatggaa aaatgaagaa tactcaagac
      781 gcaggacata cattcgtgac tt
//
