LOCUS       sequence-70019-e        1128 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses FLAG epitope tagged IFT57 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Howard PW, Jue SF, Maurer RA
  TITLE     Interaction of mouse TTC30/DYF-1 with multiple intraflagellar 
            transport complex B proteins and KIF17.
  JOURNAL   Exp Cell Res. 2013 Jun 25. pii: S0014-4827(13)00271-1. doi: 
            10.1016/j.yexcr.2013.06.010.
  PUBMED    23810713
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp Cell 
            Res. 2013 Jun 25. pii: S0014-4827(13)00271-1. doi: 
            10.1016/j.yexcr.2013.06.010."
COMMENT     Sequence Label: FLAG-IFT57-pcDNA3
FEATURES             Location/Qualifiers
     source          1..1128
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      134..143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             161..184
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttggtaccgg gccccccctc gaggtcgacg
      121 cggccgcaag cttgccacca tggggggcgc gcaggctagc gactacaagg acgacgatga
      181 caaggcacat atggccgccg cggccgcggt gatcccgccg tcgggcttgg acgatggggt
      241 gtctcgggct cgcggggaag gcgcagggga ggctgtggtg gagcgcgggc caggagcggc
      301 ctaccacatg ttcgtggtga tggaagactt agtggagaag ctgaagctgc tccgctacga
      361 ggaggagcta ctccgaaaga gcaatctgaa gcccccgtcc agacactact ttgctctgcc
      421 taccaaccca ggcgagcagt tctacatgtt ttgcactctt gctgcgtggc tgatcaacaa
      481 aactggccgt gcctttgagc agcctcaaga atacgacgat cccaatgcaa ctatatctaa
      541 tatactctct gagcttcgct cttttgggag aactgcagat tttcctcctt caaaattaaa
      601 gtctggttac ggagaacaag tgtgctatgt tcttgattgc ttagctgaag aagctttaaa
      661 atatattggt ttcacttgga aaaggccatc atacccagtg gaagaactag aagaagaaac
      721 tgttccagaa gatgatgccg agttaacatt aagtaaagtg gatgaagaat ttgtggaaga
      781 ggagacagat aatgaagaaa actttattga tctcaacgtt ttaaaggccc agacctatcg
      841 cttggacaca aacgagtctg ccaaacaaga agatattttg gaatctacga cagatgctgc
      901 ggaatggagc ctagaagttg agcgtgtact accgcagctg aaagtcacga ttaggactga
      961 caataaggat tggaggatcc atgttgacca aatgcaccag cacaaaagtg ggattgaatc
     1021 tgctctgaag gagaccaagg ggtttttgga caagctccat aatgaaatta gcaggactct
     1081 ggaaaagatt ggcagccgag aaaagtacat taacaatcaa cttgagca
//
