LOCUS       sequence-70021-e        1007 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for Ca2+ inside primary cilia.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Su S, Phua SC, Derose R, Chiba S, Narita K, Kalugin PN, Katada T, 
            Kontani K, Takeda S, Inoue T
  TITLE     Genetically encoded calcium indicator illuminates calcium dynamics 
            in primary cilia.
  JOURNAL   Nat Methods. 2013 Sep 22. doi: 10.1038/nmeth.2647.
  PUBMED    24056873
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 22. doi: 10.1038/nmeth.2647."
COMMENT     Sequence Label: 5HT6-GCaMP5G
FEATURES             Location/Qualifiers
     source          1..1007
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(38..481)
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMKYTDSEEEIREAFRVFDKDGNGYISAAEL
                     RHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     primer_bind     597..616
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     856..877
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(983..1004)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agancggtgg anctgtcgac tgcagagttc gaagctttca cttcgctgtc atcatttgta
       61 caaactcttc gtagtttacc tgaccatccc catcgatgtc tgcttccctg atcatttcat
      121 caacctcttc atctgttaac ttctctccaa ggtttgtcat cacgtggcga agctctgctg
      181 cactgatgta gccattgcca tccttatcaa acacacggaa cgcttctcta atttcttctt
      241 cactgtctgt gtatttcatt tttcttgcca tcattgtcag gaactcaggg aagtcgattg
      301 tgccgtcacc gtcggcatct acttcattga tcatgtcctg cagctctgct tctgtggggt
      361 tctgccccag agaccgcatc accgtcccca gctccttggt tgttattgtc ccatccccgt
      421 ccttgtcaaa tagggagaaa gcctctttaa attctgcgat ctgctcttca gtcagttggt
      481 ccggcaggtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc
      541 ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct
      601 tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gatgtagcct tcgggcatgg
      661 cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag cactgcacgc
      721 cgtaggtcag ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga
      781 tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc accctcgccg gacacgctga
      841 acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca
      901 gctcctcgcc cttgctcacc atgctccctc cggtaccgcc cttgtacagc tcgtccatgc
      961 cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtga
//
