LOCUS       sequence-70023-e         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET reporter for Ca2+ inside primary cilia.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Su S, Phua SC, Derose R, Chiba S, Narita K, Kalugin PN, Katada T, 
            Kontani K, Takeda S, Inoue T
  TITLE     Genetically encoded calcium indicator illuminates calcium dynamics 
            in primary cilia.
  JOURNAL   Nat Methods. 2013 Sep 22. doi: 10.1038/nmeth.2647.
  PUBMED    24056873
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 22. doi: 10.1038/nmeth.2647."
COMMENT     Sequence Label: TNXXL-CTS20
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     34..58
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      174..183
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(180..182,183..185,186..698)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     primer_bind     complement(225..246)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(486..505)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     833..854
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agnnnnncnt atnagtgnta gagnncgtcg acgagctcgt ttagtgaacc gtcagatcgc
       61 ctggagacgc catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct
      121 ccggactcta gcgtttaaac ttaagcttgg taccgagctc ggatcccgcg gccgccacca
      181 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
      241 gcgacgtaaa cggccacaag ttcagcgtgc gcggcgaggg cgagggcgat gccaccaacg
      301 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
      361 tcgtgaccac cctgacctgg ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
      421 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgt accatcttct
      481 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
      541 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
      601 agctggagta caactacatc agccacaacg tctatatcac cgccgacaag cagaagaacg
      661 gcatcaaggc ccacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
      721 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
      781 acctgagcac ccagtccaag ctgagcaaag accccaacga gaagcgcgat cacatggtcc
      841 tgctggagtt cgtgaccgcc gcccgcatgc tgagcgagga agagctggcc aactgcttcc
      901 gcatcttcga caaggacgct aacgggttca tcgacatcga ggagctgggt gagattctca
      961 gggccactgg ggagcacgtc accgag
//
