LOCUS       sequence-70025-e        1138 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses FLAG epitope-tagged short form of mouse KIF17 in mammalian
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1138)
  TITLE     FLAG-KIF17-Short-pcDNA3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: FLAG-KIF17-short-pcDNA3
FEATURES             Location/Qualifiers
     source          1..1138
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      93..102
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             120..143
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttgccaccat ggggggcgcg caggctagcg
      121 actacaagga cgacgatgac aaggcacata tggcctcgga gtcagtgaaa gtggtcgtgc
      181 gctgccgacc catgaacaag cgcgagcgcg agctgagctg ccagtcggtg gtgacggtgg
      241 acagtgcgcg cggccaatgc ttcattcaga acccgggagc ggcggacgaa cccccaaagc
      301 agtttacctt cgatggcgcc tactacatag aacacttcac cgagcagatc tacaacgaga
      361 tcgcctaccc gctggtggag ggtgtcaccg aaggctacaa tgggaccatt tttgcctatg
      421 gccagacagg cagtgggaag tctttcacca tgcagggact gccagatccc ccctgccaga
      481 ggggcatcat tcccagagcc ttcgagcacg tgtttgagag cgttcagtgt gcagagaaca
      541 ccaagttctt agtgcgggct tcctatctgg agatctacaa tgaagatgtc catgacctgc
      601 tgagtgctga caccaagcag aggcttgagc tgaaggagca tcccgagaag ggagtgtatg
      661 tcaaggggct gtccatgcac acggtacaca acgtagccca gtgtgagcgc gtcatggaga
      721 ccggctggaa gaaccgtgcg gttggctaca ccctgatgaa taaggactcc tcccgttccc
      781 actccatctt taccatcaac atcgagatct atgccgtgga tgaacggggt aaggaccatc
      841 ttcgcgcagg caagctcaac ctggtagacc tggccggcag tgagcggcaa tccaagactg
      901 gggccacagg ggaaaggctc aaggaggcca ccaaaattaa cctgtcactg tcggcgctgg
      961 gcaacgtcat ctcagcactg gtggatgggc gctgcaagca cattccttac cgggactcga
     1021 agctgacccg actgcttcag gactcactgg gtggcaacac caagactctg atggtggctt
     1081 gcctgtctcc cgcagacaac aattacgatg agaccctcag tacgctgcgc tatgctaa
//
